食品科学 ›› 2006, Vol. 27 ›› Issue (8): 144-148.

• 工艺技术 • 上一篇    下一篇

牛初乳中类胰岛素生长因子的提纯研究

 贾建波, 李相前, 朱礼尧   

  1. 淮阴工学院生物工程系; 淮安市第四人民医院
  • 出版日期:2006-08-15 发布日期:2011-10-14

Study on Purification of Bovine Colostrums Insulin-like Growth Factors

 JIA  Jian-Bo, LI  Xiang-Qian, ZHU  Li-Yao   

  1. 1.Department of Biological Engineering,Huaiyin Institute of Technology,Huaian 223001,China;2.Huaian Fourth Hospital,Huaian 223001,China
  • Online:2006-08-15 Published:2011-10-14

摘要: 本文研究了从牛初乳中用中空纤维超滤膜分离纯化牛初乳类胰岛素生长因子(IGF-I与IGF-Ⅱ),并利用放射免疫测定法(RIA)测定IGF-I与IGF-Ⅱ的浓度。结果表明:在碱性条件下超滤有利于提高类胰岛素生长因子的回收量。

关键词:  , 牛初乳, 类胰岛素生长因子(IGFs), 超滤, 提纯

Abstract: The purification of bovine colostrums insulin-like growth factors with the dialysis and polyacrylonitrile hollow fiber ultra-filtration membrane was studied.The density of IGF-I and IGF-Ⅱ could be assayed through radio-immunoassay(RIA).The results showed that the recovery of insulin-like growth factors(IGFs)is optimal on alkaline diafiltration.

Key words: bovine colostrums, insulin-like growth factors(IGFs), ultra-filtration, purification