食品科学 ›› 2004, Vol. 25 ›› Issue (11): 158-160.

• 工艺技术 • 上一篇    下一篇

营养因子对重组大肠杆菌产β-胡萝卜素的影响

 邹祥, 廖志华, 廖国建, 胡昌华, 孙敏   

  1. 西南师范大学生命科学学院现代生物医药研究所
  • 出版日期:2005-11-15 发布日期:2011-10-24

Effects of Nutrient Factors onβ-carotene Production of Recombinant Escherichia coli

 ZOU  Xiang, LIAO  Zhi-Hua, LIAO  Guo-Jian, HU  Chang-Hua, SUN  Min   

  1. Institute of Modern Biopharmaceuticals, Southwest China Normal University
  • Online:2005-11-15 Published:2011-10-24

摘要: 研究了各种营养因子对重组大肠杆菌产β-胡萝卜素的影响,以葡萄糖10g/L和蔗糖10g/L为复合碳源,牛肉膏4g/L为氮源有利于菌体生长和β-胡萝卜素的表达,添加微量因子NaCl、Span-20和KH2PO4有利于β-胡萝卜素的表达,而对细胞生长作用不明显,最佳添加浓度分别为1.6、0.7和3g/L。

关键词: 营养因子, 重组大肠杆菌, &beta, -胡萝卜素

Abstract: In this paper, the nutrient factors onβ-carotene production of recombinant Escherichia coli were studied. Compoundcarbon sources, glucose 10g/L and sucrose 10g/L, and beef extract 4 g/L used as nitrogen sources were favor for cell growth andβ-carotene expression. Addition NaCl1.6g/L,Span-20 0.7g/L and KH2PO4 3g/L helped forβ-carotene expression., But hadno effect on cell growth.

Key words: nutrient factors, recombinant Escherichia coli, β-carotene