食品科学 ›› 2003, Vol. 24 ›› Issue (10): 56-59.

• 基础研究 • 上一篇    下一篇

血红蛋白酶法水解过程数学模型的建立

 张建国, 周建勇, 罗飞, 程文新   

  1. 双汇集团技术中心
  • 出版日期:2003-10-15 发布日期:2011-12-13

The Building of Mathematical Model for the Process of Hydrolyzing Haemoglobin by Protease

 ZHANG  Jian-Guo, ZHOU  Jian-Yong, LUO  Fei, CHENG  Wen-Xin   

  1. Technology Center of Shineway Group
  • Online:2003-10-15 Published:2011-12-13

摘要: 采用响应面分析法(RSM)对血红蛋白酶解过程中的酶解温度、酶解pH和酶与底物的比值([E]/[S])等因素进行优化,并建立了血红蛋白酶解过程的数学模型。考虑到成本和工艺要求等问题,最终确定酶解工艺为[E]/[S]:0.0048AU/g、酶解pH值:7.0和酶解温度:55℃

关键词: 血红蛋白, 水解, 数学模型

Abstract: In this article, response surface analysis method was used to optimize conditions of hydrolyzing haemoglobin byprotease, and the mathematical model for the process of hydrolyzing haemoglobin was established. At last, for the reasons ofboth technics and cost. The optimal conditions were pH7.0, 55℃and enzyme/substrate ratio 0.0048AU/g.

Key words: haemoglobin, hydrolysis, mathematical model