抗副溶血弧菌IgY的制备及活性检测

食品科学 ›› 2008, Vol. 29 ›› Issue (12): 469-472.

抗副溶血弧菌IgY的制备及活性检测

钟青萍, 王斌, 蒲俊, 何彬斌

华南农业大学食品学院；广州市华南农大生物药品有限公司；

出版日期:2008-12-15 发布日期:2011-12-08

Production of Yolk Immunoglobin against Vibrio parahaemololyticus and Detection of Antibody Activity

ZHONG Qing-Ping, WANG Bin, PU Jun, HE Bin-Bin

1.College of Food Science,South China Agricultural University,Guangzhou 510642,China; 2.Guangzhou South China Biological Medicine Co. Ltd.,Guangzhou 510642,China

Online:2008-12-15 Published:2011-12-08

摘要/Abstract

摘要： 副溶血性弧菌(Vibrio parahaemololyticus,Vp)是我国微生物食源性疾病的主要病因,且是海水养殖业中的常见病原菌,造成巨大的经济损失。本研究制备副溶血弧菌免疫原,并以5×107、5×108CFU/ml两种剂量分别免疫母鸡,获得特异性的抗副溶血弧菌IgY;并建立ELISA方法检测免疫鸡蛋中的抗体效价,高剂量免疫组的抗体效价可达1:57600,低剂量免疫组的抗体效价达1:28800;低剂量组抗体效价可维持250d,高剂量组抗体效价可维持300d以上。

关键词: 副溶血性弧菌, IgY, 制备, 活性检测

Abstract: Vibrio parahaemololyticus is the main cause of foodborne disease,and it is also a common pathogen detected from seafood. Vibrio parashaemoloyticus would cause enormous loss when fish and shrimp and so on are infected by it. In this study,laying hens were divided into two groups,and were immunized with two different doses of prepared antigen. The low dose group of hens was immunized with 5×107 CFU/ml,and the high dose group of hens was immunized with 5×108 CFU/ml Vibrio parahaemololyticus. ELISA was established to detect the antibody activity. The results demonstrated that the highest titer of IgY against Vibrio parahaemololyticus in the high and low dose group could reach 1:57600 and 1:28800,respectively. The titer of IgY produced by the low dose group could maintain for 250 days,but the titer of IgY produced by the high dose group could maintain for more than 300 days.

Key words: Vibrio parahaemololyticus, IgY, production, antibody activity

钟青萍, 王斌, 蒲俊, 何彬斌. 抗副溶血弧菌IgY的制备及活性检测[J]. 食品科学, 2008, 29(12): 469-472.

ZHONG Qing-Ping, WANG Bin, PU Jun, HE Bin-Bin. Production of Yolk Immunoglobin against Vibrio parahaemololyticus and Detection of Antibody Activity[J]. FOOD SCIENCE, 2008, 29(12): 469-472.

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