食品科学 ›› 2003, Vol. 24 ›› Issue (4): 34-38.

• 基础研究 • 上一篇    下一篇

木瓜蛋白酶降解壳聚糖动力学研究

 黄永春, 李琳, 郭祀远, 蔡妙颜, 黎海彬   

  1. 华南理工大学轻化工研究所
  • 出版日期:2003-04-15 发布日期:2011-12-13

Study on Hydrolysis Kinetics of Chitosan by Papain

 HUANG  Yong-Chun, LI  Lin, GUO  Si-Yuan, CAI  Miao-Yan, LI  Hai-Bin   

  • Online:2003-04-15 Published:2011-12-13

摘要: 研究了木瓜蛋白酶催化壳聚糖降解过程中,温度、pH值、酶量、底物浓度等条件对降解速度的影响。结果表明,反应最适pH和温度分别为4.5和45℃左右,降解速度随着用酶量的增加而线性增加,降解过程符合米氏方程。

关键词: 壳聚糖, 木瓜蛋白酶, 降解, 动力学

Abstract: The effects of reaction temperature, pH, enzyme concentration and ch itosan concentration on the hydrolysis of chitosans by papain were studied. It showed that the optimum temperature and pH were 50℃ and pH4.5 respectively. Th e reaction velocity was increased while the enzyme concentration raised. Finall y , the kinetic equation was established.

Key words: chitosan, papain, depolymerization, kinetics