不同培养条件对诱导Pseudomonas syringae高表达冰核活性蛋白的影响

食品科学 ›› 2000, Vol. 21 ›› Issue (12): 23-26.

不同培养条件对诱导Pseudomonas syringae高表达冰核活性蛋白的影响

陈庆森, 高秀芝, 张亚琳, 阎亚丽, 刘剑虹, 庞广昌

天津商学院生物技术与蛋白资源研究室；

出版日期:2000-12-15 发布日期:2011-12-05

Effects of Different Culture Conditions on High-Level Expression of the Ice-nuclei Proteins of Pseudomonas Syringae

CHEN Qing-Sen, GAO Xiu-Zhi, ZHANG Ya-Lin, YAN Ya-Li, LIU Jian-Hong, PANG Guang-Chang

Online:2000-12-15 Published:2011-12-05

摘要/Abstract

摘要： 对冰核活性细菌(Pseudomonas syringae QF-95-F19)在不同条件下高表达冰核蛋白特性进行了研究。采用22℃单温培养和32℃-18℃双温培养的方法，优选出了一种高表达冰核活性蛋白的M1培养基。考察了不同温度对诱导冰核蛋白的影响，通过在28℃、32℃培养后的低温诱导，证实了低温是QF-95-F19适应环境诱导冰核蛋白表达的必要条件。从不同pH对QF-95-F19高表达冰核蛋白的影响，确定了pH与冰核蛋白形成及细菌生长势之间的关系。实验证实了不同蛋白抑制剂对QF-95-F19冰核蛋白的表达具有显著抑制作用。

关键词: 冰核细菌, 冰核活性蛋白, 双温培养方法, 抑制剂, 诱导

Abstract: The ice-nucleation active (INA) bacteria (Pseudomonas syringae QF-95-F19) of the high-level expression ice-nuclei feature under the different conditions was studied in this paper. Culture methods were used in 22℃ and 32℃shifted to 18℃ as two segment temperature cultures , TSTC with the optimal growth medium(M1) as the basic medium to do the experiment. The effects of different temperatures on high-level expression of ice-nuclei were detected assayed in the M1. Experiment results confirmed that low temperature was a required factor for expressing ice-nuclei in TSTC and ice nucleation protein became necessary . From the effects of the different pH on the high-level expression ice nuclei to QF-95-F19, the relationship between the pH and ice nuclei tromation and the growth situation of the bacterium was determined further. It confirmed that the different inhibitors had inhibition remarkably for the expression of QF-95-F19 ice nuclei and its growth by this experiment.

Key words: Ice-nucleation active (INA)bacteria Ice-nuclei Two segment temperature culture (TSTC) method Inhibitor Induction

陈庆森, 高秀芝, 张亚琳, 阎亚丽, 刘剑虹, 庞广昌. 不同培养条件对诱导Pseudomonas syringae高表达冰核活性蛋白的影响[J]. 食品科学, 2000, 21(12): 23-26.

CHEN Qing-Sen, GAO Xiu-Zhi, ZHANG Ya-Lin, YAN Ya-Li, LIU Jian-Hong, PANG Guang-Chang. Effects of Different Culture Conditions on High-Level Expression of the Ice-nuclei Proteins of Pseudomonas Syringae[J]. FOOD SCIENCE, 2000, 21(12): 23-26.

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