食品科学 ›› 2007, Vol. 28 ›› Issue (5): 177-181.

• 工艺技术 • 上一篇    下一篇

响应面优化提高大豆分离蛋白中蛋白干基得率的工艺研究

 张丽华, 张东杰, 张桂芳   

  1. 黑龙江八一农垦大学食品学院; 黑龙江八一农垦大学食品学院 黑龙江大庆163319; 黑龙江大庆163319;
  • 出版日期:2007-05-15 发布日期:2011-12-31

Study on Optimizing Technology with Response Surface Methodology to Enhance Dry Protein Content of Soy Isolate Protein

 ZHANG  Li-Hua, ZHANG  Dong-Jie, ZHANG  Gui-Fang   

  1. College of Food Science,Heilongjiang August First Land Reclaimanation University,Daqing 163319,China
  • Online:2007-05-15 Published:2011-12-31

摘要: 为获得更高含量大豆分离蛋白干基,采用物理沉降法,控制酸沉pH梯度变化,添加氯化钠调整溶液离子强度,逐级分离沉降11S大豆蛋白和7S大豆蛋白,以提高大豆分离蛋白质干基含量。分别在料液比、温度、NaCl加入量的单因素试验基础上,进行响应面优化设计分析。结果表明最佳工艺条件为料液比1:11.35、萃取温度48.63℃、NaCl加入量0.126mol/L,获得蛋白质干基含量96.04%,同比市售较好水平产品93.31%的蛋白质干基含量提高了2.67%。

关键词: 大豆分离蛋白, 蛋白干基含量, 响应面, 工艺

Abstract: In order to obtain high dry protein content of soy isolate protein,using the physical methods controlling pH gradient to change acid precipitating step,and adding sodium chloride to alter ion intensity of solution,in order to separating 11S and 7S orderly.Therefore,it could enhance the dry protein content of soy isolate protein.On the basis of single factor test of material fluid proportion,temperature,sodium chloride addition,response surface optimization design was carried on to enhance the dry protein content of soy isolate protein on the most superior parameter condition.The result indicates the best condition that material fluid proportion is 1:11.35,the extract temperature is 48.63℃,NaCl addition is 0.126 mol/L,which leads to the dry protein content of soy isolate protein to achieve 96.04%,and is better than the market products that is 93.31% to increasing 2. 67%.

Key words: soy isolate protein, dry protein content, response surface methodology, technology