微波法提取紫苏黄酮类物质及其成分分析

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 53-57.

微波法提取紫苏黄酮类物质及其成分分析

张蕾蕾，常雅宁*，夏鹏竣，俞建瑛，徐炎鹏

华东理工大学生物工程学院

收稿日期:2011-09-06 修回日期:2012-10-20 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 常雅宁 E-mail:changyn@ecust.edu.cn
基金资助:
上海市科委重大应用专项

Microwave-Assisted Extraction and Composition Analysis of Flavonoids from Perilla frutescens Leaves

Received:2011-09-06 Revised:2012-10-20 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 研究微波法提取紫苏黄酮类物质的最佳工艺条件。采用单因素及响应面优化试验，分别考察紫苏粒径、提取时间、微波温度、乙醇体积分数及料液比对提取率的影响。结果表明：紫苏粒径20目、提取时间70min、温度71℃、乙醇体积分数61%、料液比1:20(g/mL)时，黄酮得率最高为59.28mg/g。紫苏提取液经柱层析法纯化后，经全波长扫描和高效液相色谱显示，该物质主要成分为芦丁、木犀草素等。

关键词: 微波, 紫苏, 黄酮类物质, 响应面优化, 柱层析, 全波长扫描, 高效液相色谱法

Abstract: Response surface methodology was used to optimize conditions for the microwave-assisted extraction of flavonoids from Perilla frutescens leaves. The effects of raw material particle size, extraction time, temperature, ethanol concentration and solid-to-solvent ratio on extraction efficiency were investigated. The optimal conditions for extracting flavonoids from Perilla frutescens leaves were found to be extraction at 71 ℃ for 70 min with 61% ethanol at a solid-to-solvent ratio of 1:20 (g/mL) and a raw material particle size of 20 mesh. Under these conditions, the maximum extraction yield of flavonoids was 59.28 mg/g. The obtained extract was purified by D152 macroporous resin column chromatography and the purified product mainly consisted of lutin and luteolin as demonstrated by UV absorption spectrometry and HPLC.

Key words: microwave, Perilla frutescens, flavonoids, response surface methodology, column chromatography, full wavelength scan, high performance liquid chromatography (HPLC)

中图分类号:

TS202.1

张蕾蕾，常雅宁*，夏鹏竣，俞建瑛，徐炎鹏. 微波法提取紫苏黄酮类物质及其成分分析[J]. 食品科学, 2012, 33(22): 53-57.

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