胶体金免疫层析技术应用于食品中黄原胶

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 270-273.

胶体金免疫层析技术应用于食品中黄原胶

夏敏1，杜美红1，贾瑜2，赵建业1，陈舜琮1，张德红2，赵正苗2

1.北京市理化分析测试中心 2.北京勤邦生物技术有限公司

收稿日期:2011-09-16 修回日期:2012-09-22 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 夏敏 E-mail:xiamin91@yahoo.com.cn
基金资助:
北京市科研院科技创新工程项目“产品质量安全与创新方法重点实验室建设（三期）”

Detection of Xanthan Gum in Foods by Colloid-Gold Immunochromatographic Assay

Received:2011-09-16 Revised:2012-09-22 Online:2012-10-25 Published:2012-11-09
Contact: Min XIA E-mail:xiamin91@yahoo.com.cn

摘要/Abstract

摘要： 目的：建立一种简便实用的胶体金免疫层析检测新方法，用于食品中黄原胶的快速筛查。方法：用黄原胶抗原标准品免疫新西兰大白兔，制备得到高效价的抗黄原胶多克隆抗体；采用柠檬酸三钠还原法制备胶体金，并将其与抗黄原胶多克隆抗体标记制得金标抗体；在硝酸纤维素膜检测线上，预包被黄原胶标准抗原，质控线上包被羊抗兔IgG，采用竞争法，制成黄原胶胶体金免疫层析检测试纸条，并对试纸条的特异性、敏感性和稳定性进行了试验。结果：该试纸条检测样品的敏感性为3g/kg。结论：研制出黄原胶胶体金免疫层析试纸条及其检测方法，该方法敏感性高、特异性强、操作简便，5min可出检测结果，可用于现场大量样品的快速检测。

关键词: 黄原胶, 胶体金免疫层析, 快速检测

Abstract: Objective: To develop a new rapid colloid-gold immunochromatographic assay for the screening of xanthan gum in foods. Methods: The immunochromatographic assay was based on competitive immunoassay. Standard xanthan gum was used to immunize New Zealand rabbits to obtain high titer polyclonal antibodies. Colloid-gold was prepared according to the sodium citrate method, and was then conjugated to polyclonal antibodies. Xanthan gum antigen was coated on nitrocellulose membrane and goat anti-rabbit IgG was coated on control line. Results: The developed colloid-gold immnunochromatographic assay revealed a detection sensitivity of 3 g/kg. Conclusion: A new colloid-gold immunochromatographic strip for the detection of xanthan gum has been successfully developed. This method is highly specific, sensitive, rapid (the detection procedure can be completed in 5 min) and suitable for in situ, rapid, large-scale detection.

Key words: xanthan gum, colloid-gold immunochromatography, rapid screening

中图分类号:

TS207.3

夏?敏1，杜美红1，贾?瑜2，赵建业1，陈舜琮1，张德红2，赵正苗2. 胶体金免疫层析技术应用于食品中黄原胶[J]. 食品科学, 2012, 33(20): 270-273.

参考文献

[1] 食品整治办〔2008〕3号《食品中可能违法添加的非食用物质和易滥用的食品添加剂品种名单（第一批）》的通知.
[2] 姜艳彬，孙冠如，王海等.盐酸克伦特罗胶体金快速检测试纸条的研制[J].中国畜牧兽医,2010,37(10):109-113.
[3] 孙洋，冯书章，郭学军等.肠出血性大肠杆菌O157胶体金免疫层析试纸条的研制[J].中国人兽共患病学报,2008,24(4):356-359.
[4] 赵海锋，甘一如. 胶体金免疫层析法检测小分子物质[J].农药,2007,46(7): 439-446.
[5] 崔焕忠，张辉，王兴龙. 胶体金试纸快速检测食品中单增李斯特菌[J].食品科学,2010,31(4): 239-242.
[6] LAMBERT L A，WAMER W G，WEI R R ,et al. The protective but nonsynergistic effect of dietary β-carotene and vitamin E on skin tumorigenesis in skh mice[J].Nutrition and cancer,1994,21(1):1.
[7] 刘晓波，蔡美英，王霞等. 一种简单实用纯化腹水McAb方法—辛酸/硫酸铵法[J].华西医大学报,1999,30(4): 455-456.
[8] 杨利国,胡少昶,魏平华等.酶免疫测定技术[M].南京:南京大学出版社,1998.272-273.
[9] XIULAN S，XIAOLIAN Z，JIAN T，et al.Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxinB1[J].Int J Food Microbiol,2005,99(2):185-194.
[10] MIKA P A，LAITINEN M P，VUENTO M. Affinity immunosensor for milk progoesterrone: identification of critical parameter[J].Biosensors and Bioelectronica,1996,11(12):1207-1214.
[11] 张改平,职爱民,邓瑞广等.兽药残留的免疫学快速检测技术概述[J].河北农业科学,2009,(9):193-195.
[12] 王钱，李康，陈树芬等.胶体金免疫层析法对组织样品中磺胺甲噁唑残留的快速检测[J].分析测试学报,2009,28(4):458-461.
[13] 张慧嫦，张少恩，吴忠华等.胶体金免疫层析法快速检测盐酸克伦特罗残留[J].中国国境卫生检疫杂志,2008,31(1):39-42.
[14] 林晓丽，饶辉，熊勇华等.胶体金试纸条快速检测食品中磺胺二甲基嘧啶残留[J].食品科学,2010,31(24):366-369.
[15] 向军俭，王五洲，唐勇等.胶体金免疫层析法检测食用肉中的氨基脲[J].中国国境卫生检疫杂志,2008,31(1):39-42.