屎肠球菌冷冻干燥保护剂筛选及稳定性比较

食品科学 ›› 2013, Vol. 34 ›› Issue (3): 239-242.

屎肠球菌冷冻干燥保护剂筛选及稳定性比较

彭虹旎¹,梁莉¹,陈西广²

1. 青岛康地恩生物科技有限公司技术中心
2. 中国海洋大学海洋生命学院

收稿日期:2011-10-18 修回日期:2013-01-05 出版日期:2013-02-15 发布日期:2017-12-29
通讯作者: 彭虹旎 E-mail:penghongni@gmail.com

Selection of Cryoprotectants for Enterococcus faecium in freeze-drying and Storage Stability

Received:2011-10-18 Revised:2013-01-05 Online:2013-02-15 Published:2017-12-29

摘要/Abstract

摘要： 屎肠球菌是人及动物肠道中正常菌群的一部分，对肠系菌群具有调控作用。屎肠球菌在微生态产品中的应用效果受产品活菌量、贮存稳定性影响明显，对其研究十分必要。通过正交试验设计，及4℃、37℃贮存稳定性比较，优化适合屎肠球菌的冻干保护剂配方为：脱脂乳5%、蔗糖1%、麦芽糊精5%。采用此保护剂配方制备的屎肠球菌冻干样品，在铝箔袋密封包装、温度37℃、相对湿度75%条件下存放4周，菌存活率为97.90%。

关键词: 屎肠球菌, 冷冻干燥, 保护剂, 微生态

Abstract: Enterococcus faecium is one part of normal microflora of human and animal intestinal tract, the bacteria colony has the regulative function to the intestines. The application effect of Enterococcus faecium used in probiotics is largely influenced by the number of vial bacteria and storage stability. In this study, the optimal cryoprotectant formula for Enterococcus faecium was determined using orthogonal array design and storage stability test at 4℃ and 37℃ to be composed of 5% skim milk,1% sucrose and 5% maltodextrin. The survival rate of Enterococcus faecium was 97.90% after 4 weeks, which was protected in sealed aluminum foil bag, under the condition of 37℃ and relative humidity 75%.

Key words: Enterococcus faecium, freeze-drying, croprotectant, probiotic

中图分类号:

TS202.3

彭虹旎梁莉陈西广. 屎肠球菌冷冻干燥保护剂筛选及稳定性比较[J]. 食品科学, 2013, 34(3): 239-242.

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