食品科学 ›› 2019, Vol. 40 ›› Issue (10): 70-76.doi: 10.7506/spkx1002-6630-20180530-419

• 生物工程 • 上一篇    下一篇

Enterococcus faecium AS8及其胞外多糖对发酵乳流变学特性的影响

白 英,刘乃齐   

  1. 内蒙古农业大学食品科学与工程学院,内蒙古 呼和浩特 010018
  • 出版日期:2019-05-25 发布日期:2019-05-31
  • 基金资助:
    国家自然科学基金地区科学基金项目(31760460)

Rheological Properties of Fermented Milk as Affected by Addition of Enterococcus faecium AS8 and Its Exopolysaccharides

BAI Ying, LIU Naiqi   

  1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Online:2019-05-25 Published:2019-05-31

摘要: 通过红外光谱、气相色谱-质谱联用和流变仪的检测,探究产自屎肠球菌(Enterococcus faecium)AS8的胞外多糖(exopolysaccharide,EPS)(AS8-EPS)的结构组成和流变性能。采用3 种发酵乳作为样品,分别为Streptococcus thermophilus+Lactobacillus bulgaricus、EPS+S. thermophilus+L. bulgaricus和E. faecium AS8。结果表明,在贮藏期间,不同发酵乳样品具有不同的流变学性质。同时,补充添加EPS和原位EPS对发酵乳流变性能具有不同的影响。通过Sephadex G-100和Sephadex G-50的分离纯化,获得2 种多糖,分别为AS8-1-EPS和AS8-2-EPS。AS8-1-EPS主要单糖组成为甘露糖、葡萄糖和半乳糖(分别占59.1%、26.8%、7.9%),还有含量很少的其他单糖;AS8-2-EPS主要单糖组成为甘露糖、葡萄糖、半乳糖和鼠李糖(分别占65.4%、21.3%、8.9%、4.4%)。红外光谱检测结果表明AS8-1-EPS和AS8-2-EPS均为杂多糖。

关键词: 胞外多糖, 屎肠球菌, 结构组成, 流变学特性

Abstract: This study aimed to investigate the structural composition of exopolysaccharides (EPS) from Enterococcus faecium AS8 and the rheological properties of traditional fermented dairy products in Inner Mongolia containing respectively Streptococcus thermophiles + Lactobacillus bulgaricus, EPS + S. thermophiles + L. bulgaricus and E. faecium AS8. Two fractions of EPS, named as AS8-1-EPS and AS8-2-EPS, were obtained by Sephadex G-100 column chromatography and purified by Sephadex G-50 column chromatography. AS8-1 was mainly composed of mannose (59.1%), glucose (26.8%) and galactose (7.9%). AS8-2-EPS was mainly composed of mannose (65.4%), glucose (21.3%), galactose (8.9%) and rhamnose (4.4%). The results of Fourier transform infrared (FT-IR) spectroscopy revealed that both EPS fractions were heteropolysaccharides.

Key words: exopolysaccharide, Enterococcus faecium, structural composition, rheological properties

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