食品科学 ›› 2013, Vol. 34 ›› Issue (8): 100-103.doi: 10.7506/spkx1002-6630-201308020

• 工艺技术 • 上一篇    下一篇

正交试验优化酶法提取菜籽皮不溶性膳食纤维工艺

王顺民 郑锐   

  1. 安徽工程大学生物与化学工程学院,安徽 芜湖 241000
  • 收稿日期:2011-12-26 修回日期:2013-01-21 出版日期:2013-04-25 发布日期:2013-05-07
  • 通讯作者: 王顺民 E-mail:wangshunmin@126.com
  • 基金资助:
    安徽省优秀青年人才基金项目

Optimization of Enzymatic Extraction of Insoluble Dietary Fiber from Rapeseed Shells

WANG Shun-min,ZHENG Rui   

  1. College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China
  • Received:2011-12-26 Revised:2013-01-21 Online:2013-04-25 Published:2013-05-07
  • Contact: shun-min WANG E-mail:wangshunmin@126.com

摘要: 目的:以菜籽皮为原料,研究不溶性膳食纤维的酶法提取工艺条件。方法:采用淀粉酶和蛋白酶酶解菜籽皮,以不溶性膳食纤维得率为指标,通过正交试验优化最佳工艺条件。结果:淀粉酶加酶量0.7%,料液比1:20、pH5.5、温度40℃、酶解时间60min,在此条件下菜籽不溶性膳食纤维得率为81.24%;蛋白酶的添加量0.7%、料液比1:20、pH7.5、酶解温度40℃、酶解时间60min,在此条件下菜籽不溶性膳食纤维得率为77.13%。结论:确定了影响膳食纤维提取的主要影响因素,得到了菜籽皮不溶性膳食纤维酶解法提取的最佳条件。

关键词: 菜籽皮, 不溶性膳食纤维, 酶法, 提取

Abstract: The enzymatic extraction of insoluble dietary fiber (IDF) from rapeseed shells was optimized by orthogonal array design based on IDF yield. The IDF yield from rapeseed shells was 81.24% after 60 min of amylase hydrolysis at 40 ℃ and an initial pH of 5.5 with an enzyme dosage of 0.7% and a solid-to-liquid ratio of 1:20, and 77.13% after 60 min of protease hydrolysis at 40 ℃ and an initial pH of 7.5 with an enzyme dosage of 0.7% and a solid-to-liquid ratio of 1:20. In this study, we have identified main process parameters that influence IDF extraction from rapeseed shells and established the optimum process conditions.

Key words: rapeseed shell, insoluble dietary fiber, enzymatic method, extraction

中图分类号: