萝卜苗中异硫氰酸盐的提取工艺

doi:10.7506/spkx1002-6630-201308006

食品科学 ›› 2013, Vol. 34 ›› Issue (8): 27-30.doi: 10.7506/spkx1002-6630-201308006

萝卜苗中异硫氰酸盐的提取工艺

赵功玲1，郝睿2，由宏2，朱毅2,*，罗云波2

1.河南科技学院食品学院，河南新乡 453003；2.中国农业大学食品科学与营养工程学院，北京 100083

收稿日期:2011-12-03 修回日期:2013-02-04 出版日期:2013-04-25 发布日期:2013-05-07
通讯作者: 赵功玲 E-mail:hnzgl@163.com
基金资助:
国家自然基金项目

Extraction of Isothiocyanate from Radish Sprout

ZHAO Gong-ling1，HAO Rui2，YOU Hong2，ZHU Yi2,*，LUO Yun-bo2

1. School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China；2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received:2011-12-03 Revised:2013-02-04 Online:2013-04-25 Published:2013-05-07

摘要/Abstract

摘要： 以正己烷和二氯甲烷为溶剂，4-methylthio-3-butenyl isothiocyanate(MTBITC)和4-methylsulfinyl-3-butenyl isothiocyanate(Sulforaphene)的总量为提取效率研究萝卜苗中异硫氰酸盐的提取工艺。结果表明：提取萝卜苗中异硫氰酸盐的最佳工艺条件为自身酶解时间3～5min、液固比2:1(mL/g)、按正己烷-二氯甲烷-二氯甲烷的溶剂顺序各提取1次、提取时间3min/次。

关键词: 萝卜苗, 提取, 异硫氰酸盐, 4-methylthio-3-butenyl isothiocyanate(MTBITC), 4-methylsulfinyl-3-butenyl isothiocyanate(Sulforaphene)

Abstract: Total content of MTBITC and sulforaphene was considered as a parameter to evaluate extract efficiency, extraction technology of isothiocyanate from radish sprout was carried out using n-hexane and dichloromethane being extraction solvent. The optimal extraction conditions were: enzymolysis for 3 min, a liquid/solid ratio of 2 mL/g, an extraction time of 3-5 min, and n-hexane, dichloromethane, dichloromethane being extract order in turn.

Key words: radish sprouts, extract, isothiocyanates, 4-methylthio-3-butenyl isothiocyanate (MTBITC), 4-methylsulfinyl-3-butenyl isothiocyanate (Sulforaphene)

中图分类号:

TS201.4

赵功玲1，郝睿2，由宏2，朱毅2,*，罗云波2. 萝卜苗中异硫氰酸盐的提取工艺[J]. 食品科学, 2013, 34(8): 27-30.

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萝卜苗中异硫氰酸盐的提取工艺

Extraction of Isothiocyanate from Radish Sprout

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