微波辅助法提取蓝莓多糖BBP0-2的分离纯化及组分分析

doi:10.7506/spkx1002-6630-201312025

摘要/Abstract

摘要：

采用微波辅助法从蓝莓中提取多糖，比较传统的双氧水法脱色和三氯乙酸-正丁醇法脱蛋白与聚酰胺柱层析法脱色脱蛋白的效果，蓝莓粗多糖经DEAE-52纤维素离子交换柱和Sephadex G-100凝胶柱分离纯化，应用高效液相色谱法鉴定纯度，气相色谱法分析单糖组成。结果表明：通过响应面优化设计确定最佳工艺条件为料液比1:8(g/mL)、微波功率600W、微波提取时间30min，此条件下提取量达到3.32%，聚酰胺柱层析法脱色脱蛋白效果明显优于传统方法。蓝莓多糖经DEAE-52纤维素离子交换柱分离得到3个级分BBP0、BBP1、BBP2，BBP0经SephadexG-100凝胶柱进一步纯化得到蓝莓多糖BBP0-2组分，主要由阿拉伯糖、半乳糖、木糖和葡萄糖4种单糖组成，其物质的量比为2:5:3:4。

关键词: 蓝莓, 多糖, 微波提取, 分离纯化

Abstract:

The microwave-assisted extraction of polysaccharide from blueberry fruit was optimized by respons surface
methdology in this work. Hydrogen peroxide, trichloroacetic acid-n-butanol and polyamide column chromatography were
compared for their efficiencies in removing pigment and protein from crude polysaccharide from blueberry fruit. After
further purification by DEAE-cellulose 52 column and Sephadex G-100 column chromagraphy, we analyzed the purity
by HPLC and monosaccharide composition of purifed polysaccharide by GC. The optimal conditions for polysaccharide
extraction were found to be 1:8 (g/mL), 600 W and 30 min for solid/solvent ratio, microwave power and extraction time,
respectively. The extraction efficiency of ploysaccharide under the optimized conditions was 3.32%. Polyamide column
chromatography was considerably more effective than the traditional methods with hydrogen peroxide and trichloroacetic
acid-n-butanol. Three polysaccharides were separated from DEAE-cellulose 52 column chromatography, namely BBP0,
BBP1 and BBP2. BBP0 were further separated by Sephadex G-100 column chromagraphy to obtain BBP0-2, which was
composed of arabinose, galactose, xylose and glucose with a molar ratio of 2:5:3:4.

Key words: blueberry, polysaccharide, microwave-assisted extraction, isolation and purification

孟宪军，常瑜，孙希云*，刘晓晶，沈昳潇. 微波辅助法提取蓝莓多糖BBP0-2的分离纯化及组分分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201312025.

MENG Xian-jun，CHANG Yu，SUN Xi-yun*，LIU Xiao-jing，SHEN Yi-xiao. Microwave-Assisted Extraction, Purification and Identification of Polysaccharide BBP0-2 from Blueberry Fruit[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312025.

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微波辅助法提取蓝莓多糖BBP0-2的分离纯化及组分分析

Microwave-Assisted Extraction, Purification and Identification of Polysaccharide BBP0-2 from Blueberry Fruit

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