双歧杆菌BB12对便秘大鼠的通便作用及对胃肠激素的调节

doi:10.7506/spkx1002-6630-201613037

摘要/Abstract

摘要：

目的：研究双歧杆菌BB12对便秘大鼠的通便作用及对相关胃肠激素的调节。方法：将48 只Wistar大鼠按性别分开，雌雄各半。按体质量随机分为正常对照组、阳性对照组、模型对照组、双歧杆菌BB12低、中、高剂量组，每组4 只。造模后灌胃相应受试样品，记录各组大鼠每日进食量、饮水量、体质量、排便粒数，测定粪便含水量、小肠炭末推进率、血清胃泌素、胃动素、P物质、生长抑素含量。结果：双歧杆菌BB12能显著减少便秘大鼠的饮水量，增加便秘大鼠的排便粒数、小肠炭末推进率、血清胃动素和P物质含量，降低生长抑素含量，且作用具有一定的性别差异和剂量相关性。对便秘大鼠的进食量、体质量、粪便含水量、血清胃泌素含量无明显作用。结论：双歧杆菌BB12对便秘大鼠具有通便作用，其作用机制与其调节胃肠激素的分泌有关。

关键词: 双歧杆菌, 便秘, 胃肠激素

Abstract:

Objective: To investigate the effects of Bifidobacterium BB12 on promoting defecation and regulating
gastrointestinal hormones in constipated rats. Methods: Totally 48 Wistar rats, half male and half female, were divided
randomly into control group, positive control group, model group, Bifidobacterium BB12 low-, middle-, and high-dose
groups. Rat models of loperamide hydrochloride-induced constipation were established and the rats from each group were
treated with corresponding drugs. During the experiment, daily food intake, water intake, body weight, fecal number, fecal
water content, gastrointestinal transit, and the contents of gastrin, motilin, substance P and somatostatin in serum were
measured. Results: Fecal number, gastrointestinal transit, and the serum contents of motilin and substance P increased
significantly, while water intake and somatostatin content decreased significantly in response to the administration of
Bifidobacterium BB12. These effects were dependent on gender and dose. There was no significant difference in food intake,
body weight, fecal water content and serum gastrin. Conclusion: The laxative effect of Bifidobacterium BB12 is confirmed
and the underlying mechanism is related to regulating some gastrointestinal hormones.

Key words: Bifidobacterium, constipation, gastrointestinal hormone

中图分类号:

R33
TS201.3

田颖，戴倩倩，时明慧，孟婵芳，黄玉军，顾瑞霞. 双歧杆菌BB12对便秘大鼠的通便作用及对胃肠激素的调节[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613037.

TIAN Ying, DAI Qianqian, SHI Minghui, MENG Chanfang, HUANG Yujun, GU Ruixia. Bifidobacterium BB12 Promotes Defecation and Regulates Gastrointestinal Hormones in Constipated Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613037.

参考文献

[1] NELLESEN D, YEE K, CHAWLA A, et al. A systematic review of the

economic and humanistic burden of illness in irritable bowel syndrome

and chronic constipation[J]. Journal of Managed Care Pharmacy, 2013,

19(9): 755-764. DOI:10.18553/jmcp.2013.19.9.755.

[2] FAVRETTO D C, PONTIN B, MOREIRA T R. Effect of the

consumption of a cheese enriched with probiotic organisms

(Bifidobacterium lactis bi-07) in improving symptoms of

constipation[J]. Arquivos de Gastroenterologia, 2013, 50(3): 196-201.

DOI:10.1590/S0004-28032013000200035.

[3] 唐伟峰, 唐晓军, 杨巍. 功能性便秘的中西医研究进展[J]. 世界中西

医结合杂志, 2015, 10(6): 880-884. DOI:10.13935/j.cnki.sjzx.150643.

[4] 叶飞, 王巧民. 慢性便秘的流行病学研究进展[J]. 中国临床保健杂

志, 2010, 13(6): 665-667.

[5] 贡鸣, 李岩. 功能性便秘的治疗现状[J]. 中国当代医药, 2009, 16(16):

199-200.

[6] DIMIDI E, CHRISTODOULIDES S, FRAGKOS K C, et al. The

effect of probiotics on functional constipation in adults: a systematic

review and meta-analysis of randomized controlled trials[J]. American

Journal of Clinical Nutrition, 2014, 100: 1075-1084. DOI:10.3945/

ajcn.114.089151.

[7] QUIGLEY E M M. The enteric microbiota in the pathogenesis

and management of constipation[J]. Best Practice & Research

Clinical Gastroenterology, 2011, 25(1): 119-126. DOI:10.1016/

j.bpg.2011.01.003.

[8] 邹华军, 张其圣, 石磊, 等. 直投式乳酸菌剂对便秘模型小鼠肠道功

能的影响[J]. 食品与发酵科技, 2013, 49(5): 26-29.

[9] DIMIDI E, CHRISTODOULIDES S, FRAGKOS K C, et al. The

effect of probiotics on functional constipation in adults: a systematic

review and meta-analysis of randomized controlled trials[J]. American

Journal of Clinical Nutrition, 2014, 100(4): 1075-1084. DOI:10.3945/

ajcn.114.089151.

[10] MILLER L E, OUWEHAND A C. Probiotic supplementation

decreases intestinal transit time: meta-analysis of randomized

controlled trials[J]. World Journal of Gastroenterology, 2013, 19(29):

4718-4725. DOI:10.3748/wjg.v19.i29.4718 .

[11] PITKALA K H, STRANDBERG T E, FINNE SOVERI U H, et al.

Fermented cereal with specific Bifidobacteria normalizes bowel

movements in elderly nursing home residents. A randomized,

controlled trial[J]. The Journal of Nutrition, Health & Aging, 2007,

11(4): 305-311.

[12] 孙建华, 刘晓放, 于丽萍. 双歧杆菌BB12发酵乳的润肠通便功效研

究[J]. 黑龙江医药, 2013, 26(2): 214-217.

[13] 中华人民共和国国家卫生和计划生育委员会. 可用于婴幼儿食品的

菌种名单[EB/OL]. (2011-12-20)[2015-07-10]. http://www.moh.gov.cn/

sps/s7891/201111/a10fe4a0b1dd477c9884649220368cc2.shtml.

[14] JUNGERSEN M, WIND A, JOHANSEN E, et al. The Science

behind the probiotic strain Bifidobacterium animalis subsp.

lactis BB-12[J]. Microorganisms, 2014, 2: 92-110. DOI:10.3390/

microorganisms2020092.

[15] 彭芝榕, 刘晓梅, 倪学勤, 等. 植物乳杆菌F1208对便秘大鼠胃肠

道功能的影响[J]. 食品科学, 2013, 34(13): 243-246. DOI:10.7506/

spkx1002-6630-201313051.

[16] BUSTOS D, OGAWA K, PONS S, et al. Effect of loperamide and

bisacodyl on intestinal transit time, fecal weight and short chain fatty

acid excretion in the rat[J]. Acta Gastroenterologica Latinoamericana,

1991, 21(1): 3-9.

[17] WINTOLA O A, SUNMONU T O, AFOLAYAN A J. The effect

of Aloe ferox Mill. in the treatment of loperamide-induced

constipation in Wistar rat[J]. BMC Gastroenterology, 2010, 10: 95.

DOI:10.1186/1471-230x-10-95.

[18] 陶国枢, 余颂涛, 张玲, 等. 新型止泻药洛哌丁胺治疗老年人腹泻疗

效及其药理研究[J]. 老年学杂志, 1990, 10(3): 145-147.

[19] 许履正, 徐妙娣. 洛哌丁胺的药理作用和临床应用[J]. 新药与临床,

1988, 7(4): 196-198.

[20] MCCREA G L, MIASKOWSKI C, STOTTS N A, et a1. A review

of the literature on gender and age differences in the prevalence

and characteristics of constipation in North America[J]. Journal

of Pain Symptom Manage, 2009, 37: 737-745. DOI:10.1016/

j.jpainsymman.2008.04.016.

[21] 辛海威, 方秀才, 柯美云. 功能性便秘性别差异及其原

因[ J ] . 协和医学杂志, 2 0 11 ( 3 ) : 281-284. DOI:10.3969/

j.issn.1674-9081.2011.03.019.

[22] 王欢, 黄永坤, 刘梅. 胃肠激素与胃肠道功能及疾病的关

系[J]. 医学综述, 2013, 19(15): 2735-2738. DOI:10.3969/

j.issn.1006-2084.2013.15.016.

[23] 陆英杰, 连至诚. 胃肠激素对胃肠动力的影响[J]. 免疫学杂志, 2006,

22(3): 94-96.

[24] SUO H Y, ZHAO X, QIAN Y, et al. Therapeutic effect of activated

carbon-Induced constipation mice with Lactobacillus fermentum Suo

on treatment[J]. International Journal of Molecular Sciences, 2014,

15(12): 21875-21895. DOI:10.3390/ijms151221875.

[25] 索静宇, 徐华. 胃肠动力的影响因素[J]. 临床消化病杂志, 2013,

25(4): 249-250. DOI:10.3870/lcxh.j.issn.1005-541X.2013.04.21.