云南晒青红茶与烘青红茶香气成分对比

doi:10.7506/spkx1002-6630-201614011

摘要/Abstract

摘要：

采用顶空固相微萃取（headspace solid-phase microextraction，HS-SPME）结合气相色谱-质谱（gaschromatography-mass spectrometry，GC-MS）分析3 种晒青红茶和3 种烘青红茶的香气成分，并利用主成分分析（principal component analysis，PCA）和聚类分析（clustering analysis，CA）找出这2 种不同干燥方式红茶的异同点，以实现对它们的区分。结果表明，2 种红茶中一共鉴定出香气成分76 种，香气成分主要以醇类、酮类和酯类化合物为主，其中醇类化合物相对含量在晒青红茶和烘青红茶中分别占57.43%和60.45%，主要包括芳樟醇、芳樟醇氧化物、香叶醇和橙花叔醇等萜烯醇类物质。通过对比发现，晒青红茶和烘青红茶在香气成分的组成及相对含量上存在较高程度的相似性。但通过使用PCA和CA，晒青红茶和烘青红茶之间能实现较好的区分，说明不同的干燥方式对这2 种红茶的香气成分有一定的影响。因此，通过结合晒青红茶和烘青红茶的香气成分及相对含量，HSSPME-GC-MS结合多元统计学方法能够实现2 种不同干燥方式红茶的区分。

关键词: 晒青红茶, 烘青红茶, 香气成分, 气相色谱-质谱, 多元统计学方法

Abstract:

The aroma components of sun-dried and baked black teas, three replicates each, extracted with headspace solidphase
microextraction (HS-SPME) were identified by gas chromatography-mass spectrometry (GC-MS). Their similarities
and differences were compared by using principal component analysis (PCA) and clustering analysis (CA). A total of
76 aroma components were identified in these two kinds of black tea, mainly including alcohols, ketones, and esters
compounds. Among them, alcohol compounds were the most abundant components in sun-dried black tea (57.43%) and
baked black tea (60.45%), mainly including linalool, linalool oxides, geraniol, nerolidol, etc. The aroma components of sundried
and baked black teas were very similar and difficult to distinguish from each other. However, PCA and CA showed
that sun-dried and baked black teas could be clearly distinguished according to their volatile characteristics, which illustrated
that drying methods have influences on aroma components of black tea. Therefore, HS-SPME-GC-MS in combination with
multivariate statistical methods could provide a feasible and rapid technique to differentiate between sun-dried and baked
black teas based on their volatile components and relative contents.

Key words: sun-dried black tea, baked black tea, aromat components, gas chromatography-mass spectrometry (GC-MS), multivariate statistical methods

中图分类号:

TS272

吕世懂，吴远双，王晨，高雪梅，李江兵，张文睿，孟庆雄. 云南晒青红茶与烘青红茶香气成分对比[J]. 食品科学, doi: 10.7506/spkx1002-6630-201614011.

Lü Shidong, WU Yuanshuang, WANG Chen, GAO Xuemei, LI Jiangbing, ZHANG Wenrui, MENG Qingxiong. Comparative Study of Volatile Components in Sun-Dried and Baked Black Teas in Yunnan Province[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614011.

参考文献

[1] KUMAZAWA K, MASUDA H. Change in the flavor of black tea

drink during heat processing[J]. Journal of Agricultural and Food

Chemistry, 2001, 49(7): 3304-3309. DOI:10.1021/jf001323h.

[2] STODT U W, BLAUTH N, NIEMANN S, et al. Investigation of

processes in black tea manufacture through model fermentation

(oxidation) experiments[J]. Journal of Agricultural and Food

Chemistry, 2014, 62(31): 7854-7861. DOI:10.1021/jf501591j.

[3] 廉明, 吕世懂, 吴远双, 等. 我国4 种红茶的挥发性成分分析[J].

热带亚热带植物学报, 2015, 23(3): 301-309. DOI:10.11926/

j.issn.1005-3395.2015.03.011.

[4] 黄振宇. 滇红茶传统工艺技术与现代设备技术的应用[J]. 现代园艺,

2012(2): 32-33. DOI:10.3969/j.issn.1006-4958.2012.02.021.

[5] 刘顺航, 赵甜甜, 李相波, 等. 不同制作工艺对滇红茶感官品质及

理化性质的影响[J]. 湖南农业科学, 2014(10): 66-68. DOI:10.3969/

j.issn.1006-060X.2014.20.021.

[6] 刘素强, 杨娟, 袁林颖, 等. 烘焙提香条件对红茶感官品质及主要生

化成分的影响[J]. 食品安全质量检测学报, 2015, 6(4): 1301-1306.

[7] KUMAR R S S, MURUGESAN S, KOTTUR G, et al. Black tea: the

plants, processing/manufacturing and production[J]. Tea in Health

and Disease Prevention, 2013, 2013: 41-57. DOI:10.1016/B978-0-12-

384937-3.00004-5.

[8] 吕世懂, 吴远双, 姜玉芳, 等. 不同产区乌龙茶香气特征及差异分析[J]. 食品

科学, 2014, 35(2): 146-153. DOI:10.7506/spkx1002-6630-201402027.

[9] LÜ S, WU Y, ZHOU J, et al. The study of fingerprint characteristics

of Dayi pu-erh tea using a fully automatic HS-SPME/GC-MS and

combined chemometrics method[J]. PloS One, 2014, 9(12): e116428.

DOI:10.1371/journal.pone.0116428.

[10] 任洪涛, 周斌, 夏凯国, 等. 不同级别云南工夫红茶香气成分比

较分析[J]. 南方农业学报, 2012, 43(4): 489-492. DOI:10.3969/

j:issn.2095-1191.2012.04.489.

[11] 王倩, 张立芹, 叶能胜, 等. 基于HS-SPME/GC-MS与模式识别技术判别

红茶产地[J]. 首都师范大学学报(自然科学版), 2011, 32(6): 42-47.

[12] 赖幸菲, 庞式, 李裕南, 等. 不同季节翠玉品种茶叶香气组

分的GC-MS分析[J]. 现代食品科技, 2014, 30(12): 287-293.

DOI:10.13982/j.mfst.1673-9078.2014.12.048.

[13] 周斌, 任洪涛, 秦太峰, 等. 不同提取方法在滇红香气成分分析中

的对比研究[J]. 现代食品科技, 2011, 27(7): 850-855. DOI:10.3969/

j.issn.1673-9078.2011.07.031.

[14] LÜ S, WU Y, LI C, et al. Comparative analysis of Pu-erh and Fuzhuan

teas by fully automatic headspace solid-phase microextraction coupled

with gas chromatography-mass spectrometry and chemometric

methods[J]. Journal of Agricultural and Food Chemistry, 2014, 62(8):

1810-1818. DOI:10.1021/jf405237u.

[15] LIN J, DAI Y, GUO Y, et al. Volatile profile analysis and quality

prediction of Longjing tea (Camellia sinensis) by HS-SPME/GCMS[

J]. Journal of Zhejiang University Science B, 2012, 13(12): 972-

980. DOI:10.1631/jzus.B1200086.

[16] LÜ H P, ZHONG Q S, LIN Z, et al. Aroma characterisation of Pu-erh

tea using headspace-solid phase microextraction combined with GC/

MS and GC-olfactometry[J]. Food Chemistry, 2012, 130(4): 1074-

1081. DOI:10.1016/j.foodchem.2011.07.135.

[17] DU L, LI J, LI W, et al. Characterization of volatile compounds of puerh

tea using solid-phase microextraction and simultaneous distillationextraction

coupled with gas chromatography-mass spectrometry[J].

Food Research International, 2014, 57: 61-70. DOI:10.1016/

j.foodres.2014.01.008.

[18] QIAO Y, XIE B, ZHANG Y, et al. Characterization of aroma active

compounds in fruit juice and peel oil of Jinchen sweet orange fruit

(Citrus sinensis (L.) Osbeck) by GC-MS and GC-O[J]. Molecules,

2008, 13(6): 1333-1344. DOI:10.3390/molecules13061333.

[19] PANG X, QIN Z, ZHAO L, et al. Development of regression model

to differentiate quality of black tea (Dianhong): correlate aroma

properties with instrumental data using multiple linear regression

analysis[J]. International Journal of Food Science and Technology,

2012, 47(11): 2372-2379. DOI:10.1111/j.1365-2621.2012.03112.x.

[20] SCHUH C, SCHIEBERLE P. Characterization of the key aroma compounds

in the beverage prepared from Darjeeling black tea: quantitative differences

between tea leaves and infusion[J]. Journal of Agricultural and Food

Chemistry, 2006, 54(3): 916-924. DOI:10.1021/jf052495n.

[21] BORSE B B, JAGAN MOHAN RAO L, NAGALAKSHMI S, et

al. Fingerprint of black teas from India: identification of the regiospecific

characteristics[J]. Food Chemistry, 2002, 79(4): 419-424.

DOI:10.1016/S0308-8146(02)00191-7.

[22] WACHE Y, BOSSER-DERATULD A, LHUGUENOT J C, et al.

Effect of cis/trans isomerism of β-carotene on the ratios of volatile

compounds produced during oxidative degradation[J]. Journal

of Agricultural and Food Chemistry, 2003, 51(7): 1984-1987.

DOI:10.1021/jf021000g.

[23] ALASALVAR C, TOPAL B, SERPEN A, et al. Flavor characteristics

of seven grades of black tea produced in Turkey[J]. Journal of

Agricultural and Food Chemistry, 2012, 60(25): 6323-6332.

DOI:10.1021/jf301498p.

[24] TOLDRA F, FLORES M, SANZ Y. Dry-cured ham flavour:

enzymatic generation and process influence[J]. Food Chemistry, 1997,

59(4): 523-530. DOI:10.1016/S0308-8146(97)00013-7.

[25] WANG L F, LEE J Y, CHUNG J O, et al. Discrimination of teas

with different degrees of fermentation by SPME-GC analysis of the

characteristic volatile flavour compounds[J]. Food Chemistry, 2008,

109(1): 196-206. DOI:10.1016/j.foodchem.2007.12.054.

[26] MA C, DASTMALCHI K, FLORES G, et al. Antioxidant and

metabolite profiling of North American and neotropical blueberries

using LC-TOF-MS and multivariate analyses[J]. Journal of

Agricultural and Food Chemistry, 2013, 61(14): 3548-3559.

DOI:10.1021/jf400515g.

[27] LÜ S D, WU Y S, SONG Y Z, et al. Multivariate analysis based on

GC-MS fingerprint and volatile composition for the quality evaluation

of Pu-erh green tea[J]. Food Analytical Methods, 2015, 8(2): 321-333.

DOI:10.1007/s12161-014-9900-0.

[28] MOREDA-PINEIRO A, FISHER A, HILL S J. The classification of

tea according to region of origin using pattern recognition techniques

and trace metal data[J]. Journal of Food Composition and Analysis,

2003, 16(2): 195-211. DOI:10.1016/S0889-1575(02)00163-1.

[29] LÜ S, WU Y, WEI J, et al. Application of gas chromatography-mass

spectrometry and chemometrics methods for assessing volatile profiles

of Pu-erh tea with different processing methods and ageing years[J].

RSC Advances, 2015, 107: 87806-87817. DOI:10.1039/C5RA15381F.