超高效液相色谱-串联质谱法检测鸡肉和鸡肝中抗病毒药物利巴韦林残留

doi:10.7506/spkx1002-6630-201620024

食品科学 ›› 2016, Vol. 37 ›› Issue (20): 146-150.doi: 10.7506/spkx1002-6630-201620024

超高效液相色谱-串联质谱法检测鸡肉和鸡肝中抗病毒药物利巴韦林残留

齐凯1,2，汤晓艳1,2,*，陶瑞2，杨小体1,2，张小庆2，沈习习1,2

1.南京农业大学食品科技学院，国家肉品质量安全控制工程技术研究中心，江苏南京 210095；
2.中国农业科学院农业质量标准与检测技术研究所，农业部农产品质量安全重点实验室，北京 100081

收稿日期:2016-10-27 修回日期:2016-10-27 出版日期:2016-10-25 发布日期:2016-12-01
通讯作者: 汤晓艳
基金资助:
中国农业科学院科技创新工程项目

Determination of Residues of the Antiviral Drug Ribavirin in Chicken Muscle and Liver by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometryx

QI Kai, TANG Xiaoyan, TAO Rui, YANG Xiaoti, ZHANG Xiaoqing, SHEN Xixi

1. National Centry of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University,
Nanjing 210095, China; 2. Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Institute of Quality Standards
and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01
Contact: TANG Xiaoyan

摘要/Abstract

摘要：

为研究禁用抗病毒药物利巴韦林在鸡组织中的残留规律，对京红蛋鸡以30 mg/kg体质量剂量连续口服灌喂，在不同的时间点采集鸡肉和鸡肝样品，以超高效液相色谱-串联质谱法检测利巴韦林含量。对鸡肉、鸡肝中利巴韦林检测的方法学考察表明，鸡肉中利巴韦林检测方法线性相关系数R2＞0.99，检出限为1.20 μg/kg，质量浓度范围为5.0～200 μg/L；鸡肝中利巴韦林检测方法线性相关系数R2＞0.98，检出限为1.54 μg/kg，质量浓度范围为10.0～500 μg/L；高、中、低3 种添加水平下回收率为83.5%～120.6%，精密度（相对标准偏差）为0.7%～9.1%，满足残留分析方法要求。利巴韦林口服灌喂蛋鸡后被迅速代谢吸收，在鸡肝中代谢消除较快，残留时间短，最长检出时间为24 h，即超过24 h不再有检出，鸡肉中利巴韦林消除速率相对较慢，残留时间较长，最长检出时间为60 h，在两种组织中均不发生蓄积现象。

关键词: 鸡肉, 鸡肝, 利巴韦林, 超高效液相色谱-串联质谱法, 残留规律

Abstract:

This study aimed to investigate the distribution prolfiles of residues of the antiviral drug ribavirin in chicken
tissues, whose use has been banned. Ribavirin at the dose of 30 mg/kg was continuously administered to Jinghong laying
hens. The animals were executed and chicken muscle and liver samples were collected at different time intervals for the
detection of ribavirin content by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The
calibration curve for ribavirin in chicken muscle was linear in the concentration range of 5.0–200 μg/L with a correlation
coefficients (R2) of above 0.99, and the limit of detection (LOD) was 1.20 μg/kg. The calibration curve for ribavirin in
chicken liver was linear in the concentration range of 10.0–500 μg/L with a correlation coefficients (R2) of above 0.98, and
the the limit of detection (LOD) was 1.54 μg/kg. At three spiked concentration levels, the recoveries of ribavirin ranged from
83.5% to 120.6%, and the precision expressed as relative standard deviation (RSD) ranged from 0.7%–9.1%, which met
the basic quantitative requirements. The results showed that ribavirin in chicken liver was rapidly eliminated and the final
detection time was 24 h, while the elimination rate for muscle was relatively slow, and the final detection time was 60 h after
oral administration. Finally, there was no accumulation of ribavirin residue in both tissues.

Key words: chicken muscle, liver, ribavirin, ultra performance liquid chromatography-tandem mass spectrometry, residue distribution pattern

中图分类号:

TS251.7

齐凯，汤晓艳，陶瑞，杨小体，张小庆，沈习习. 超高效液相色谱-串联质谱法检测鸡肉和鸡肝中抗病毒药物利巴韦林残留[J]. 食品科学, 2016, 37(20): 146-150.

QI Kai, TANG Xiaoyan, TAO Rui, YANG Xiaoti, ZHANG Xiaoqing, SHEN Xixi. Determination of Residues of the Antiviral Drug Ribavirin in Chicken Muscle and Liver by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometryx[J]. FOOD SCIENCE, 2016, 37(20): 146-150.

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超高效液相色谱-串联质谱法检测鸡肉和鸡肝中抗病毒药物利巴韦林残留

Determination of Residues of the Antiviral Drug Ribavirin in Chicken Muscle and Liver by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometryx

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