芸豆芽菜多酚对D-半乳糖致小鼠机体氧化及肾脏损伤的修复作用

doi:10.7506/spkx1002-6630-201713036

食品科学 ›› 2017, Vol. 38 ›› Issue (13): 219-223.doi: 10.7506/spkx1002-6630-201713036

芸豆芽菜多酚对D-半乳糖致小鼠机体氧化及肾脏损伤的修复作用

王颖，王欣卉，徐炳政，朱磊，佐兆杭，刘淑婷，宫雪

1.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；2.国家杂粮工程技术研究中心，黑龙江大庆 163319；3.青岛琅琊台集团股份有限公司，山东青岛 266400

出版日期:2017-07-15 发布日期:2017-07-11

Repairing Effect of Kidney Bean Sprout Polyphenols on D-Galactose-Induced Oxidation and Kidney Injury in Mice

WANG Ying, WANG Xinhui, XU Bingzheng, ZHU Lei, ZUO Zhaohang, LIU Shuting, GONG Xue

1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. National Coarse Cereals Engineering Research Center, Daqing 163319, China; 3. Qingdao Langyatai Co. Ltd., Qingdao 266400, China

Online:2017-07-15 Published:2017-07-11

摘要/Abstract

摘要： 为探究芸豆芽菜多酚对D-半乳糖致小鼠机体氧化及肾脏损伤的修复作用，通过腹腔注射D-半乳糖溶液构建小鼠损伤模型，连续灌胃不同剂量（低、中、高分别为20、40、60 mg/（kg·d）芸豆芽菜多酚及抗坏血酸（VC）28 d后，测定小鼠体质量、肾脏脏器系数、血清谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力、超氧化物歧化酶（superoxide dismutase，SOD）活力、丙二醛（malondialdehyde，MDA）、尿素氮（blood urea nitrogen，BUN）及血肌酐（creatinine，CR）含量，并取肾脏制作苏木精-伊红染色（hematoxylineosinstaining，HE）病理学组织切片，观察小鼠肾脏损伤及灌胃给药后的恢复情况。结果发现，相较于模型对照组小鼠，除芸豆芽菜多酚低剂量组外，其他灌胃给药组小鼠体质量及肾脏系数均有所升高，但组间并无显著性差异（P＞0.05）；其他灌胃给药组小鼠血清GSH-Px活力显著上升（P＜0.05），MDA含量显著下降（P＜0.05）；各灌胃给药组小鼠血清SOD活力均较模型对照组显著上升（P＜0.05）。各灌胃给药组（除芸豆芽菜多酚低剂量组）小鼠肾脏功能指示指标BUN及CR含量均较模型对照组小鼠显著下降（P＜0.05）；肾脏HE病理学组织切片显示，模型对照组小鼠肾脏严重受损、肾小球及肾小球囊肿大并伴有间质渗血及炎性细胞浸润现象，灌胃给药后，各灌胃给药组小鼠肾脏肾小球及肾小球囊恢复正常形态，间质渗血及炎性细胞浸润现象减少。结合各灌胃给药组检测指标及HE病理学组织切片分析得知，芸豆芽菜多酚对小鼠机体氧化及肾脏损伤修复作用与剂量呈正相关。

关键词: 芸豆芽菜多酚, D-半乳糖, 氧化损伤, 肾脏损伤, 修复

Abstract: This study attempted to explore the repairing effect of kidney bean sprout polyphenols on D-galactose-induced oxidation and kidney injury in mice. For this purpose, a mouse model of oxidative injury was established by intraperitoneal injection of D-galactose solution, and the mice were gavaged with three different doses of kidney bean sprout polyphenols (20, 40, 60 mg/(kg·d)) or VC for 28 continuous days and then they were sacrificed after administration for the measurement of body weight, kidney coefficient, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA), blood urea nitrogen (BUN) and creatinine (CR) concentrations in serum as well as the histopathological examination of kidney section using HE staining. It turned out that all the treatment groups exhibited an elevation in body weight and kidney coefficient compared with the model control group, but no significant difference was observed among the treatment groups (P > 0.05). The middle-dose and high-dose groups showed a significant increase in serum GSH-Px activity (P < 0.05) and a significant reduction in MDA concentration (P < 0.05), which was not observed for the low-dose group. The administration of kidney bean sprout polyphenols resulted in a significant increase in serum SOD activity and a significant decline in BUN (as an indicator of kidney function) and CR levels except at the low dose compared with the model control group (P < 0.05). Histopathological observation showed that the kidney of mice in the model control group was severely damaged, as indicated by enlarged glomeruli and glomerular cysts together with interstitial bleeding and inflammatory cell infiltration. The enlarged glomeruli and glomerular cysts were restored to normal morphology by kidney bean sprout polyphenols, which also mitigated interstitial bleeding and inflammatory cell infiltration. Taken together, kidney bean sprout polyphenols could dose-dependently repair oxidative stress and renal injury in mice.

Key words: kidney bean sprouts polyphenols, D-galactose, oxidative damage, kidney damage, recovery

中图分类号:

TS202.3

王颖，王欣卉，徐炳政，朱磊，佐兆杭，刘淑婷，宫雪. 芸豆芽菜多酚对D-半乳糖致小鼠机体氧化及肾脏损伤的修复作用[J]. 食品科学, 2017, 38(13): 219-223.

WANG Ying, WANG Xinhui, XU Bingzheng, ZHU Lei, ZUO Zhaohang, LIU Shuting, GONG Xue. Repairing Effect of Kidney Bean Sprout Polyphenols on D-Galactose-Induced Oxidation and Kidney Injury in Mice[J]. FOOD SCIENCE, 2017, 38(13): 219-223.

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芸豆芽菜多酚对D-半乳糖致小鼠机体氧化及肾脏损伤的修复作用

Repairing Effect of Kidney Bean Sprout Polyphenols on D-Galactose-Induced Oxidation and Kidney Injury in Mice

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