尿素包合法纯化火棘籽油亚油酸与鲨烯工艺

doi:10.7506/spkx1002-6630-201116027

食品科学 ›› 2011, Vol. 32 ›› Issue (16): 127-131.doi: 10.7506/spkx1002-6630-201116027

尿素包合法纯化火棘籽油亚油酸与鲨烯工艺

梁先长1，李加兴1，田向荣2，胡平平3，黄寿恩3，黄诚4,*

1.林产化学加工工程湖南省重点实验室 2.吉首大学生物资源与环境科学学院 3.中南林业科技大学食品科学与工程学院 4.吉首大学化学化工学院

出版日期:2011-08-25 发布日期:2011-07-26
基金资助:
湖南省高校“林产资源化学与林化产品开发”科技创新团队支持计划项目(湘教通<2010>212 号)；吉首大学研究生科研创新项目 (JGY201015)

Purification of Linoleic Acid and Squalene from Pyracantha fortuneana Seed Oil by Urea Adduction Fractionation

LIANG Xian-chang1，LI Jia-xing1，TIAN Xiang-rong2，HU Ping-ping3，HUANG Shou-en3，HUANG Cheng4,*

(1. Key Laboratory of Hunan Forest Product and Chemical Industry Engineering, Zhangjiajie 427000, China ； 2. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China； 3. College of Food Science and Engineering, Central South University of Forest and Technology, Changsha 410004, China； 4. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China)

Online:2011-08-25 Published:2011-07-26

摘要/Abstract

摘要： 以火棘籽油为原料，采用尿素包合法对其亚油酸进行纯化，并在此基础上通过柱层析分离出鲨烯。通过单因素试验、正交试验和气相色谱-质谱联用(gas chromatography-mass spectroscopy，GC-MS)分析方法，研究尿素包合过程中包合时间、包合温度及火棘籽油、尿素和乙醇之间的配比等因素对亚油酸纯度和回收率的影响。结果表明：火棘籽油在原料:尿素:乙醇＝1:2:6(g/g/mL)、－5℃条件下包合12h，其亚油酸纯度从38.40%提高到68.55%，回收率达72.23%；纯化后的火棘籽油再经柱层析分离得到鲨烯，纯度达98.16%。

关键词: 关键词：火棘, 尿素包合法, 亚油酸, 柱层析, 鲨烯, GC-MS分析

Abstract: In this work, urea adduction fractionation was used to purify linoleic acid from Pyracantha fortuneana seed oil and the purified product was further separated by silica column chromatography to obtain squalene. The effects of adduction time, adduction temperature and oil-urea-ethanol ratio on the purity and recovery rate of linoleic acid were probed by one-factor-at-a-time and orthogonal array design methods. The analysis of linoleic acid and squalene was performed by GC-MS. The results showed the optimal adduction parameters were adduction time of 12 h, adduction temperature of －5 ℃, and oil-urea-alcohol ratio of 1:2:6 (g/g/mL). Under the optimal adduction conditions, the purity of linoleic acid was increased from 38.40% to 68.55%, and the recovery rate of linoleic acid reached up to 72.23%. After separation by silica column chromatography, a squalene sample with 98.16% purity was obtained .

Key words: Pyracantha fortuneana, urea adduction fractionation, linoleic acid, column chromatography, squalene, gas chromatography-mass spectrometry (GC-MS)

中图分类号:

TS225

梁先长，李加兴，田向荣，胡平平，黄寿恩，黄诚,. 尿素包合法纯化火棘籽油亚油酸与鲨烯工艺[J]. 食品科学, 2011, 32(16): 127-131.

LIANG Xian-chang，LI Jia-xing，TIAN Xiang-rong，HU Ping-ping，HUANG Shou-en，HUANG Cheng,. Purification of Linoleic Acid and Squalene from Pyracantha fortuneana Seed Oil by Urea Adduction Fractionation[J]. FOOD SCIENCE, 2011, 32(16): 127-131.

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尿素包合法纯化火棘籽油亚油酸与鲨烯工艺

Purification of Linoleic Acid and Squalene from Pyracantha fortuneana Seed Oil by Urea Adduction Fractionation

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