食品科学 ›› 2011, Vol. 32 ›› Issue (16): 338-342.doi: 10.7506/spkx1002-6630-201116074

• 分析检测 • 上一篇    下一篇

四种蜂蜜中脂肪酸的气相色谱-质谱分析

朱晓玲1,2,朱婕妤1,2,朱 露1,2,文 红2,3,*,刘 睿1,2,*   

  1. 1. 华中农业大学食品科技学院
    2.武汉市蜂产品质量控制工程技术研究中心 3.湖北省产品质量监督检验研究院
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    武汉市科技局工程技术研究中心项目(200920137007); 中央高校基本科研业务费专项资金资助项目(200922;2010PY015);华中农业大学校SRF 项目(A059)

GC-MS Analysis of Fatty Acids in Four Kinds of Honey

ZHU Xiao-ling1,2,ZHU Jie-yu1,2,ZHU Lu1,2, WEN Hong2,3,*,LIU Rui1,2,*   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Wuhan Research Center on Quality Control Engineering of Bee Products, Wuhan 430070, China; 3. Hubei Provincial Supervision and Inspection Research Institute for Product Quality, Wuhan 430061, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 对湖北产荆条蜜、紫云英蜜、柑橘蜜、油菜蜜4个蜂蜜品种中的脂肪酸组成和含量进行分析比较。采用乙醚提取蜂蜜中的脂肪酸,再以硫酸-甲醇溶液进行甲酯化处理,通过气相色谱-质谱联用仪分离鉴定其中的脂肪酸组成和相对含量。结果表明,4种蜂蜜中共分离出14种脂肪酸,其中荆条蜜、紫云英蜜及油菜蜜中分别鉴定出8种脂肪酸,柑橘蜜中鉴定出7种脂肪酸,另外,14种脂肪酸中不饱和脂肪酸占总脂肪酸含量的45.53%~79.31%,饱和脂肪酸占总脂肪酸含量的16.68%~40.77%,4种蜂蜜中均含有棕榈酸和油酸。

关键词: 蜂蜜, 脂肪酸, 气相色谱-质谱

Abstract: The composition and contents of fatty acids in four kinds of honey such as chaste tree honey, clover honey, citrus honey and rape honey collected from Hubei province were comparatively analyzed. Fatty acids were extracted by ether and esterified with 1% sulfuric acid-methanol, and then analyzed by gas chromatography-mass spectrometry (GC-MS). Totally 14 fatty acids were identified in four kinds of honey, of which 8 were isolated from chaste tree honey, clover honey and rape honey, and 7 were isolated from citrus honey. Moreover, the content of unsaturated fatty acids accounted for 45.53%-79.31% of total fatty acids, while saturated fatty acids accounted for 16.68%-40.77 %. Palmitic acid and oleic acid were detected in each kind of honey.

Key words: honey, fatty acid, gas chromatography-mass spectrometry (GC-MS)

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