食品科学 ›› 2011, Vol. 32 ›› Issue (17): 85-88.doi: 10.7506/spkx1002-6630-201117016

• 基础研究 • 上一篇    下一篇

汉源花椒精油的化学成分分析及抑菌作用

祝瑞雪1,曾维才1,赵志峰1,*,高 鸿1,闫志农2   

  1. 1. 四川大学轻纺与食品学院
    2.四川省绿色食品发展中心
  • 收稿日期:2018-05-21 修回日期:2018-05-21 发布日期:2011-08-30

Chemical Components and Antibacterial Activity of Hanyuan Zanthoxylum bungeanum Seed Oil

ZHU Rui-xue1,ZENG Wei-cai1,ZHAO Zhi-feng1,*,GAO Hong1,YAN Zhi-nong2   

  1. (1.College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China; 2. Sichuan Green Food Development Center, Chengdu 610041, China)
  • Received:2018-05-21 Revised:2018-05-21 Published:2011-08-30

摘要: 目的:分析汉源花椒精油的化学成分,评价其对常见食品腐败菌的抑制作用。方法:采用气相色谱-质谱法(GC-MS)对汉源花椒精油的挥发性成分进行分析,通过抑菌圈实验和试管二倍稀释法对其抑菌效果、最低抑菌浓度(MIC值)和最低杀菌质量浓度(MBC值)进行测定。结果:汉源花椒精油主要含D-柠檬烯(26.55%)、芳樟醇(22.11%)、乙酸芳樟酯(14.71%)、β-蒎烯(7.39%)等成分。不同抑菌方法均表明,汉源花椒精油对供试细菌有较明显的抑制作用,其中,对金黄色葡萄球菌的抑制作用最强(MIC值和MBC值分别为1.25mg/mL和2.5mg/mL),大肠杆菌最弱(MIC值和MBC值分别为5.0mg/mL和20.0mg/mL)。结论:汉源花椒精油具有良好的抑菌作用,可作为一种天然食品防腐剂资源。

关键词: 汉源花椒, 精油, 化学成分, 抑菌作用

Abstract: Objective: To analyze the chemical composition and evaluate the antibacterial activity of Hanyuan Zanthoxylum bungeanum seed oil. Methods: The chemical composition of Hanyuan Zanthoxylum bungeanum seed oil was determined by gas chromatography-mass spectrometry (GC-MS). Its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined by agar diffusion method and tube double dilution method. Results: The major components of Hanyuan Zanthoxylum bungeanum seed oil were D-limonene (26.55%), linalool (22.11%), linalyl acetate (14.71%) and β-pinene (7.39%). Meanwhile, the essential oil revealed strong antibacterial activity. Its MIC and MBC were 1.25 mg/mL and 5.0 mg/mL against Staphylococcus aureus, and 2.5 mg/mL and 20.0 mg/mL against Escherichia coli, respectively. Conclusion: Hanyuan Zanthoxylum bungeanum seed oil has excellent antibacterial activity so that it can be used as a resource of natural food preservatives.

Key words: Zanthoxylum bungeanum, essential oil, chemical components, antibacterial activity

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