非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

doi:10.7506/spkx1002-6630-201206032

食品科学 ›› 2012, Vol. 33 ›› Issue (6): 150-153.doi: 10.7506/spkx1002-6630-201206032

非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

张丽贤，周光明*，姜兰芳，许丽

发光与实时分析教育部重点实验室，西南大学化学化工学院

出版日期:2012-03-25 发布日期:2012-03-03
基金资助:
国家科技重大专项(2008ZX07315)；重庆市自然科学基金项目(CSTC，2007BB5370)

Simultaneous Determination of Chlorogenic Acid, Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography

ZHANG Li-xian，ZHOU Guang-ming*，JIANG Lan-fang，XU Li

(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)

Online:2012-03-25 Published:2012-03-03

摘要/Abstract

摘要： 目的：以5g/100mL非离子表面活性剂Triton X-100为萃取剂，建立一种简单快速的采用超声提取，高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚的方法。方法：采用Phenomenex C18色谱柱(250mm×4.6mm，5.0μm)，以甲醇-0.25%乙酸溶液为流动相梯度洗脱，流速1.0mL/min，紫外检测波长350nm。结果：绿原酸、槲皮素和山奈酚分别在0.011～220μg/mL(r＝0.9997)、0.065～130μg/mL(r＝0.9999)、0.068～136μg/mL(r＝0.9999)线性关系良好，检出限(RSN＝3)依次是1.94、0.22、0.27ng/mL；样品的加标回收率为90.40%～96.82%。结论：该方法具有样品前处理简单、绿色环保、灵敏快速等优点，可为枇杷叶中一些黄酮类物质和绿原酸的检测提供一个有效的方法。

关键词: 枇杷叶, Triton X-100表面活性剂, HPLC, 绿原酸, 槲皮素, 山奈酚

Abstract: Objective: An HPLC method for the simultaneous determination of chlorogenic acid, quercetin and kaempferol in loquat leaves was proposed based on rapid ultrasonic-assisted extraction using the non-ionic surfactant Triton X-100 as the extractant. Methods: The chromatographic separation was achieved on a Phenomenex C18 column using a mobile phase made up of methanol and 0.25% acetic acid at a flow rate of 1.0 mL/min, and the analytes were detected with a UV detector at 350 nm. Results: The calibration curves for chlorogenic acid, quercetin and kaempferol were linear in the range of 0.011－220μg/mL (r＝0.9997), 0.065－130μg/mL (r＝0.9999), 0.068－136μg/mL (r＝0.9999), respectively. The limit of detection (LOD) was 1.94, 0.22, 0.27 ng/mL (RSN＝3), respectively. The average recoveries across three spike levels ranged from 90.71% to 94.88% (n＝3). Conclusion: The method is simple, rapid, convenient, sensitive, and environment-friendly, and thus can provide a new and scientific means for assaying the contents of flavonoids and chlorogenic acid in loquat leaves.

Key words: folium eriobotryae, Triton X-100 surfactant, high performance liquid chromatography (HPLC), chlorogenic acid, quercetin, kaempferol

中图分类号:

R284.1

张丽贤，周光明，姜兰芳，许丽. 非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚[J]. 食品科学, 2012, 33(6): 150-153.

ZHANGLi-xian，ZHOUGuang-ming，JIANGLan-fang，XULi. Simultaneous Determination of Chlorogenic Acid, Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2012, 33(6): 150-153.

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非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

Simultaneous Determination of Chlorogenic Acid, Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography

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