食品科学 ›› 2012, Vol. 33 ›› Issue (8): 184-187.doi: 10.7506/spkx1002-6630-201208039

• 分析检测 • 上一篇    下一篇

流动注射抑制化学发光法快速测定婴幼儿奶瓶中的双酚A

高向阳,王长青   

  1. 河南农业大学食品科学技术学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    河南省科技攻关项目(0624430003);河南省重点学科建设基金项目(10466-X-082301)

Rapid Determination of Bisphenol A in Nursing Bottles by Flow Injection Inhibitory Chemiluminescence Analysis

GAO Xiang-yang,WANG Chang-qing   

  1. (College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 建立一种快速测定婴幼儿奶瓶双酚A含量的新型分析方法。以婴幼儿奶瓶为样品,超声波浸提后,用固相萃取柱萃取富集,利用鲁米诺-Cr3+-H2O2流动注射化学发光体系进行测定。结果表明:双酚A对该体系具有抑制发光作用,线性范围为2.0×10-9~1.0×10-5mol/L,检出限为4.6×10-10mol/L,加标回收率为95.4%~106.8%,相对标准偏差小于4%(n=7)。该法与国标法相比,具有灵敏度高、操作简便、测定快速、成本低廉、利于普及推广等优点,分析结果令人满意。

关键词: 流动注射分析(FLA),  化学发光,  鲁米诺, 奶瓶, 双酚A

Abstract: In order to establish a new rapid method for determining bisphenol A (BPA) in nursing bottles, cut pieces of nursing bottles were extracted with high-purity water under ultrasonic assistance, and the extracted analyte was enriched by solid phase extraction and determined using a uminol-H2O2-Cr chemiluminescence system. The esults showed that the chemiluminescence system was inhibited by BPA. An excellent linear relationship was observed in the range of 2.0 × 10-9 -1.0 × 10-5 mol/L. The detection limit was 4.6 × 10-10 mol/L. The average spike recoveries of BPA in 5 types of commercial samples were in the range of 95.4%-106.8%, and the relative standard deviations for 7 parallel determinations were all less than 4%. The method had the advantages of sensitivity, simplicity, rapidity, low cost and accuracy over the Chinese national standard method, thus being worth promoting and popularizing.

Key words: flow injection analysis (FIA), chemiluminescence, luminol, nursing bottle, bisphenol A

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