利用中压制备液相色谱从桑葚中快速制备矢车菊素-3-葡萄糖苷单体

doi:10.7506/spkx1002-6630-20180108-094

食品科学 ›› 2019, Vol. 40 ›› Issue (3): 94-100.doi: 10.7506/spkx1002-6630-20180108-094

利用中压制备液相色谱从桑葚中快速制备矢车菊素-3-葡萄糖苷单体

冉国敬1，蒋鑫炜1，黎浩仪2，陈俊良1，李旭升1，孙建霞2，白卫滨1,*

（1.暨南大学理工学院食品科学与工程系，广东省食品安全分子快速检测工程技术中心，广东?广州 510632；2.广东工业大学轻工化工学院，广东?广州 510090）

出版日期:2019-02-15 发布日期:2019-03-05
基金资助:
国家自然科学基金面上项目（31771983；31471588）；广州市产学研协同创新重大专项民生科技研究专项（201704020050）

Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography

RAN Guojing1, JIANG Xinwei1, LI Haoyi2, CHEN Junliang1, LI Xusheng1, SUN Jianxia2, BAI Weibin1,*

(1. Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; 2. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China)

Online:2019-02-15 Published:2019-03-05

摘要/Abstract

摘要： 单体花色苷的快速大量制备长久以来是花色苷产业化中的难题，而中压制备液相色谱在产业应用中有着很大的开发空间。选取花色苷组分较单一的桑葚为实验原料，经提取分离总花色苷后使用填装有反相C18填料的耐压玻璃柱作为中压制备液相色谱柱，纯化制备矢车菊素-3-葡萄糖苷单体。结果显示：3 个色谱分离峰中目的峰（峰2）经高效液相色谱和质谱确证为由矢车菊素-3-葡萄糖苷（cyanidin-3-glucoside，C3G）和矢车菊素-3-芸香糖苷（cyanidin-3-rutinoside，C3R）组成，采用峰面积归一化法计算得到C3G纯度为73.56%；通过对峰2采用切割方式进行收集，C3G纯度达到98%以上，单次收集到C3G单体溶液650?mL。中压制备液相色谱法单次上样量大、步骤简洁、成本低廉，可为矢车菊素-3-葡萄糖苷单体的规模化生产提供一定的参考。

关键词: 桑葚, 花色苷, 中压制备液相色谱, 矢车菊素-3-葡萄糖苷, 切割收集

Abstract: Rapid large-scale preparation of monomeric anthocyanins has always been a problem in the industrialization of anthocyanins. Preparative medium pressure liquid chromatography (MPLC) has great potential for application in the food industry. Cyanidin-3-glucoside (C3G) was purified by preparative MPLC using a C18 column from the anthocyanins extracted from mulberry fruit, containing only a few kinds of anthocyanins. The results showed that a total of three chromatographic peaks were separated. The second peak was confirmed to be composed of cyanidin-3-rutinoside (C3R) and C3G by high performance liquid chromatography (HPLC) and mass spectrometry (MS), and C3G accounted for 73.56% of the total amount as calculated by the peak area normalization method. The purity of C3G was over 98% when the peak was collected in several fractions, and a total of 650 mL of C3G was obtained in one operation. The preparative MPLC method has the advantages of large sample loading volume, simple operation and low cost and can provide a useful tool for rapid and large-scale production of C3G.

Key words: mulberry, anthocyanin, preparative medium pressure liquid chromatography, cyanidin-3-glucoside, fractional collection

中图分类号:

TS255.1

冉国敬，蒋鑫炜，黎浩仪，陈俊良，李旭升，孙建霞，白卫滨. 利用中压制备液相色谱从桑葚中快速制备矢车菊素-3-葡萄糖苷单体[J]. 食品科学, 2019, 40(3): 94-100.

RAN Guojing, JIANG Xinwei, LI Haoyi, CHEN Junliang, LI Xusheng, SUN Jianxia, BAI Weibin. Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography[J]. FOOD SCIENCE, 2019, 40(3): 94-100.

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利用中压制备液相色谱从桑葚中快速制备矢车菊素-3-葡萄糖苷单体

Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography

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