食品科学 ›› 2019, Vol. 40 ›› Issue (12): 210-219.doi: 10.7506/spkx1002-6630-20180604-034

• 成分分析 • 上一篇    下一篇

GC-TOF-MS定性和定量评估5 种木本油料种籽油的脂肪酸

丁 健1,阮成江1,*,韩 平1,Susan MOPPER2,关 莹3   

  1. 1.大连民族大学资源植物研究所,生物技术与资源利用教育部重点实验室,辽宁 大连 116600;2.路易斯安那大学生物学系,路易斯安那 拉菲特 70504-2451;3.黑龙江省农业科学院浆果研究所,黑龙江 绥棱 152200
  • 出版日期:2019-06-25 发布日期:2019-06-28
  • 基金资助:
    国家自然科学基金面上项目(31570681);国家自然科学基金青年科学基金项目(31800574);大连民族大学自主科研基金学科团队项目(0919110127);大连市科协高校创新助力联合体项目(大科协发[2018]65号)

Qualitative and Quantitative Assessment of Fatty Acids of Five Woody Crop Oils by Gas Chromatography Coupled with Time-of-Flight Mass Spectrometry (GC-TOF-MS)

DING Jian1, RUAN Chengjiang1,*, HAN Ping1, Susan MOPPER2, GUAN Ying3   

  1. 1. Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Institute of Plant Resources, Dalian Minzu University, Dalian 116600, China; 2. Department of Biology, University of Louisiana, Lafayette 70504-2451, USA; 3. Institute of Berries, Heilongjiang Academy of Agricultural Sciences, Suiling 152200, China
  • Online:2019-06-25 Published:2019-06-28

摘要: 采用气相色谱-飞行时间串联质谱和超临界二氧化碳提取技术,分析评价5 种木本油料种籽油的脂肪酸组成及相对含量。油茶和榛子种籽油中富含油酸,亚油酸是沙棘和文冠果种籽油中的主要脂肪酸,牡丹种籽油中的亚麻酸含量最高。沙棘种籽油的单不饱和脂肪酸与饱和脂肪酸比值为1.57:1,亚油酸与亚麻酸的比值为1.56:1,符合功能性健康食用油的标准;牡丹种籽油是补充人体ω-3脂肪酸的优质食用油;油茶和榛子种籽富含单不饱和脂肪酸,适合食品加工;文冠果种籽油含有3 种长链单不饱和脂肪酸(如神经酸等)。本实验评价了5 种木本油料油脂脂肪酸的组分,对指导企业生产生物活性油和人们的健康消费具有重要意义。

关键词: 木本油料油脂, 脂肪酸, 气相色谱-飞行时间串联质谱, 超临界二氧化碳提取, 定性, 定量

Abstract: Using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF-MS), we assessed the constituents and relative quantities of fatty acids in five woody seed oils extracted with supercritical carbon dioxide. Tea and hazelnut seed oils contained a high concentration of oleic acid, linoleic acid was found to be the primary fatty acid in sea buckthorn and yellow horn seed oils while the highest amount of linolenic acid occurred in peony seed oil. Sea buckthorn seed oil was considered a functional edible oil based on its ratios of monounsaturated to saturated fatty acids (1.57:1) and linoleic to linolenic acids (1.56:1). Peony seed oil was an excellent edible oil with high concentrations of omega-3 fatty acids. Tea and hazelnut seed oils were both suitable for the food industry. Interestingly, yellow horn seed oil exclusively contained three long-chain monounsaturated fatty acids (such as nervonic acid). Our study has important implications for industrial production of bioactive oils and for promoting the health of people consuming them.

Key words: woody crop oil, fatty acid, gas chromatography coupled with time-of-flight mass spectrometry, supercritical carbon dioxide extraction, identification, quantitation

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