关键词: 竹节参, 超快速液相色谱-三重四极杆飞行时间质谱法, 皂苷

Abstract: In this study, we analyzed the saponins of Panacis japonici Rhizoma by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF MS/MS). The chromatographic separation was performed on a SynergiTM Hydro-RP 100 ? (2.0 mm × 100 mm, 2.5 μm) with gradient elution using a mobile phase comprised of 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B). MS data were collected in the negative ion mode. Based on the accurate molecular mass information of quasi-molecular and fragment ions provided by highresolution mass spectrometry combined with standard reference data and relevant literature data, a total of 53 saponins were finally identified, including 18 glycosides of protopanaxadiol (PPD), 21 glycosides of protopanaxatriol (PPT), 10 glycosides of oleanolic acid and 4 glycosides of ocotillone. This study paves the way for exploring the pharmacodynamic material basis of P. japonici Rhizoma and developing a comprehensive quality evaluation system.

Key words: Panacis japonici Rhizoma, UFLC-Triple TOF MS/MS, saponins