食品科学 ›› 2019, Vol. 40 ›› Issue (23): 176-182.doi: 10.7506/spkx1002-6630-20180929-335

• 营养卫生 • 上一篇    下一篇

胡麻粕多酚对肠癌细胞凋亡的促进作用

张立超,杨宇菲,李汉卿,剌晓琴,李卓玉,   

  1. (1.山西大学生物医学研究院,山西 太原 030006;2.山西大学生物技术研究所,山西 太原 030006;3.山西大学生命科学学院,山西 太原 030006)
  • 出版日期:2019-12-15 发布日期:2019-12-24
  • 基金资助:
    国家自然科学基金面上项目(31770382;81803791);山西省重点实验室项目(201605D111004;201705D111008-21)

Apoptosis of Colon Cancer Cells Induced by Flax Meal Polyphenols

ZHANG Lichao, YANG Yufei, LI Hanqing, LA Xiaoqin, LI Zhuoyu,   

  1. (1. Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China; 2. Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; 3. School of Life Science, Shanxi University, Taiyuan 030006, China)
  • Online:2019-12-15 Published:2019-12-24

摘要: 为了探究胡麻粕多酚的肿瘤细胞抑制活性,本研究以胡麻粕为原材料,采用超声提取法和福林-酚法提取并检测提取物中的多酚质量浓度;利用细胞克隆等实验评价胡麻粕多酚对结肠癌以及正常肠上皮细胞增殖的抑制效应;借助流式细胞术和蛋白免疫印迹技术检测胡麻粕多酚对肠癌细胞凋亡的调控;并检测胞内活性氧(reactive oxygen species,ROS)水平以分析胡麻粕多酚对结肠癌细胞内ROS产生的调节作用。结果显示:胡麻粕多酚具有明显的肿瘤细胞抑制活性,并能够促进促凋亡因子Bak和Bax蛋白的表达,抑制抗凋亡因子Bcl-2和Bcl-XL蛋白的表达,且促进细胞色素c的释放;此外,随着胡麻粕多酚质量浓度的增大,DLD1和HCT-116细胞内ROS水平升高。综上所述,胡麻粕多酚能够通过提高肠癌细胞内的ROS水平并促进其进入线粒体凋亡途径从而发挥抗肿瘤作用。

关键词: 胡麻粕多酚, 结肠癌细胞, 细胞凋亡, 活性氧

Abstract: The objective of this study was to explore the anti-tumor activity of the polyphenols extracted from flax meal by ultrasonic-assisted extraction. The content of polyphenols in the extract was determined using the Folin-Ciocalteu assay. The clonogenic survival assay was used to evaluate the inhibitory effect of flax polyphenols on the proliferation of colon cancer and normal FHC cells. Flow cytometry was used to detect the apoptosis of fluorescence-labeled cells. Western blotting was used to detect the expression of apoptosis-related proteins. We also evaluated the regulation of intracellular reactive oxygen species (ROS) production by flax polyphenols. The results showed that flax meal polyphenols had obvious anti-cancer activity and pro-apoptotic activity on DLD1 and HCT-116 cells. Meanwhile, flax meal polyphenols could promote the expression of the pro-apoptotic factors Bak and Bax, inhibit the expression of the anti-apoptotic factors Bcl-2 and Bcl-XL, promote the release of cytochrome c, and elevate the level of ROS in colon cancer cells in a concentration-dependent manner. In summary, flax meal polyphenols can exert anti-tumor effects by increasing ROS level in colon cancer cells and activating mitochondrial apoptotic pathways.

Key words: flax meal polyphenols, colon cancer cell, cells apoptosis, reactive oxygen species

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