食品科学 ›› 2020, Vol. 41 ›› Issue (1): 9-15.doi: 10.7506/spkx1002-6630-20190623-270

• 基础研究 • 上一篇    下一篇

融合魏斯氏菌胞外多糖的分离纯化及其生化特性

姜静,杜仁鹏,郭尚旭,赵丹   

  1. (1.黑龙江大学生命科学学院,微生物省高校重点实验室,黑龙江 哈尔滨 150080;2.农业微生物技术教育部工程研究中心,黑龙江 哈尔滨 150080)
  • 出版日期:2020-01-15 发布日期:2020-01-19
  • 基金资助:
    国家自然科学基金面上项目(31770538);黑龙江省博士后科研启动金资助项目(LBH-Q18105)

Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa

JIANG Jing, DU Renpeng, GUO Shangxu, ZHAO Dan   

  1. (1. Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China; 2. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin 150080, China)
  • Online:2020-01-15 Published:2020-01-19

摘要: 从自制水果发酵液中分离筛选得到一株高产胞外多糖(exopolysaccharides,EPS)的乳酸菌,经生理生化实验、形态学观察及分子生物学实验鉴定该菌株为融合魏斯氏菌(Weissella confusa)。以该菌株为供试菌进行产糖发酵及分离纯化EPS,并测定EPS的化学组成、溶解性、持水性、乳化性、抑菌性及抗氧化能力。结果表明,总糖、蛋白质、糖醛酸质量分数分别为(93.67±2.08)%、(0.32±0.01)%和(6.01±0.34)%。溶解率和持水率分别为(98.78±1.37)%和(426.03±7.26)%。此外,该EPS展现出良好的乳化性、抑菌性和体外抗氧化活性,且随着EPS质量浓度的升高,作用效果越强。

关键词: 融合魏斯氏菌, 胞外多糖, 分离纯化, 生化特性

Abstract: A strain of lactic acid bacteria (LAB) with high exopolysaccharides (EPS)-producing ability was isolated from naturally fermented melon. The strain was identified as Weissella according to its physiological and biochemical properties. In addition, the chemical composition, solubility, water-holding capacity, emulsifying property, antibacterial property and antioxidant activity of the purified EPS from this strain were determined. The results showed that the total sugar, protein and uronic acid contents were (93.67 ± 2.08)%, (0.32 ± 0.01)% and (6.01 ± 0.34)%, respectively. The dissolution percentage and water holding capacity of the EPS were (98.78 ± 1.37)% and (426.03 ± 7.26)%, respectively. It exhibited good emulsifying, bacteriostatic and in vitro antioxidant properties in a concentration-dependent manner.

Key words: Weissella confusa, exopolysaccharide, separation and purification, biochemical properties

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