食品科学 ›› 2020, Vol. 41 ›› Issue (2): 101-106.doi: 10.7506/spkx1002-6630-20181228-343

• 生物工程 • 上一篇    下一篇

酸-冷交互胁迫对保护冷冻干燥发酵乳杆菌活性的作用

杨婕,郭金凤,李宝坤,卢士玲,王庆玲,董娟,蒋彩虹,姬华,王腾斌   

  1. (石河子大学食品学院,新疆植物药资源利用教育部重点实验室,新疆 石河子 832003)
  • 出版日期:2020-01-25 发布日期:2020-01-19
  • 基金资助:
    国家自然科学基金地区科学基金项目(31560444;31460007);兵团博士基金专项(2014BB005); 石河子大学重大科技攻关项目(gxjs2014-zdgg07);石河子大学高层次人才启动项目(RCZX201223); 国家自然科学基金青年科学基金项目(31201395;31301523);新疆研究生科研创新项目(XJGRI2017047)

Cryoprotective Effect of Acid-Cold Cross Stress on Lactobacillus fermentum

YANG Jie, GUO Jinfeng, LI Baokun, LU Shiling, WANG Qingling, DONG Juan, JIANG Caihong, JI Hua, WANG Tengbin   

  1. (Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Food College, Shihezi University, Shihezi 832003, China)
  • Online:2020-01-25 Published:2020-01-19

摘要: 为探究酸-冷交互胁迫对冷冻干燥发酵乳杆菌(Lactobacillus fermentum)ATm的影响,考察酸-冷交互胁迫的处理方式对细胞存活率、滞后时间以及酸化速率的影响,同时测定交互胁迫对乳酸脱氢酶、ATP酶活性以及细胞膜完整性的影响。结果表明,pH 4.5、4 ℃酸-冷交互胁迫能够显著提高细胞冷冻干燥存活率及酸化速率,其中冷冻干燥存活率能够达到87.19%,优于单因素胁迫;pH 4.5、4 ℃酸-冷交互胁迫能够提高发酵乳杆菌ATm的冷冻干燥存活率保护其活性,提高酶活性,维持细胞膜完整性。

关键词: 交互胁迫, 冷冻干燥, 发酵乳杆菌, 存活率, 活性保护

Abstract: This study aimed to explore the protective effect of acid-cold stress treatment on Lactobacillus fermentum strain ATm during freeze-drying. We determined the survival rate, lag time and acidification curve of freeze-dried bacteria cells which had been subjected to acid-cold cross stress. Furthermore, the activities of lactate dehydrogenase (LDH) and ATPase and cell membrane integrity were determined. The results showed that the acid-cold cross stress (pH 4.5 and 4 ℃) significantly improved the survival rate (reaching 87.19%, which was higher than that observed with either treatment alone) and acidification rate of the freeze-dried cells. In conclusion, acid-cold cross stress could improve the freeze-drying survival rate of L. fermentum ATm, protect its viability, increase intracellular enzyme activities and preserve cell membrane integrity.

Key words: cross-stress, freeze-drying, Lactobacillus fermentum, survival rate, viability preservation

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