食品科学 ›› 2012, Vol. 33 ›› Issue (13): 167-170.doi: 10.7506/spkx1002-6630-201213035

• 生物工程 • 上一篇    下一篇

产白藜芦醇葡萄内生菌的分离与鉴定

曾芹,师俊玲,刘延琳   

  1. 1.西北农林科技大学食品科学与工程学院 2.西北农林科技大学葡萄酒学院
  • 出版日期:2012-07-15 发布日期:2012-07-27
  • 基金资助:
    国家现代农业(葡萄)产业技术体系建设专项(nycytx-30-ch-03);农业部公益性行业(农业)科研专项(201003021)

Isolation and Identification of a Resveratrol-Producing Endophytic Fungus from Grape

ZENGQin,SHIJun-ling,LIUYan-lin   

  1. (1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China ; 2. College of Enology, Northwest A&F University, Yang ling 712100, China)
  • Online:2012-07-15 Published:2012-07-27

摘要: 从酿酒葡萄梅洛特的葡萄皮、葡萄穗轴及梗中分离出30株葡萄内生菌,经过摇瓶液体发酵后检测发酵液提取物,发现有7株内生真菌具有产白藜芦醇的能力。其中菌株MG1发酵产白藜芦醇的能力稳定、产量高,达97μg/L,其他几株菌株白藜芦醇产量较低且不稳定。对菌株MG1的分类鉴定进行重点研究,结合形态学特征及分子生物检测结果,鉴定其为链格孢属菌。

关键词: 梅洛特, 葡萄, 内生菌, 白藜芦醇, 链格孢属

Abstract: Thirty endophytic strains were isolated from the skin, cob and stem of the resveratrol-rich grape variety Merlot. Through liquid fermentation and HPLC measurement of resveratrol content in the fermentation broth, 7 capable of producing resveratrol were screened out of the 30 isolates. The strain MG1 showed the strongest stability and the highest yield in the production of resveratrol (97 μg/L), whereas other six isolates had a lower and unstable ability to produce resveratrol. The strain MG1 was identified as Alternaria sp. on the basis of its morphological characteristics and internal transcribed spacer (ITS) gene sequence.

Key words: Merlot, grape, endophyte, resveratrol, Alternaria

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