食品科学 ›› 2020, Vol. 41 ›› Issue (17): 161-168.doi: 10.7506/spkx1002-6630-20190823-250

• 营养卫生 • 上一篇    下一篇

1-脱氧野尻霉素对肥胖小鼠甲状腺激素稳态的影响

李思远,宁俊丽,丁晓雯,黄先智   

  1. (1.西南大学食品科学学院,重庆 400716;2.西南大学 家蚕基因组生物学国家重点实验室,重庆 400716)
  • 出版日期:2020-09-15 发布日期:2020-09-16
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-22)

Effect of 1-Deoxynojirimycin on Thyroid Hormone Homeostasis in Obese Mice

LI Siyuan, NING Junli, DING Xiaowen, HUANG Xianzhi   

  1. (1. College of Food Science, Southwest University, Chongqing 400716, China; 2. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China)
  • Online:2020-09-15 Published:2020-09-16

摘要: 甲状腺激素在调节脂代谢方面发挥着十分重要的作用,本实验探究肥胖对甲状腺激素稳态的影响以及1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)的干预作用。饲喂高脂饲料建立肥胖小鼠模型,继续饲喂高脂饲料的同时灌胃剂量分别为2.0、4.0、8.0 mg/(kg mb·d)的DNJ 45 d,测定血脂水平及甲状腺激素相关指标水平。结果表明,与肥胖对照组相比,高剂量DNJ能够抑制小鼠体质量的增加,改善高脂饮食引起的血脂异常;高剂量DNJ使雌、雄小鼠血清总甲状腺素水平分别上升22.03%、15.99%,总三碘甲状腺原氨酸水平分别上升66.86%、47.79%(P<0.05),促甲状腺激素水平分别下降9.65%、9.73%(P<0.05),肝脏1型脱碘酶活力分别上升40.99%、24.40%(P<0.05),肝脏1型脱碘水平分别上升35.68%、29.50%(P<0.05),肝脏甲状腺激素β受体蛋白水平分别上升37.53%、27.81%(P<0.05);并能显著上调肝脏1型脱碘酶、甲状腺激素β受体及脂代谢相关甲状腺激素靶基因脂肪甘油三酯脂酶、肉毒碱棕榈酰转移酶1α、胆固醇7α-羟化酶mRNA表达。结论:DNJ能够改善肥胖引起的甲状腺激素功能紊乱,进而上调脂代谢相关甲状腺激素靶基因mRNA表达,促进脂类分解代谢,抑制体质量增加。

关键词: 1-脱氧野尻霉素;甲状腺激素;肥胖

Abstract: Thyroid hormone plays a very important role in regulating lipid metabolism. This study explored the effect of obesity on thyroid hormone homeostasis in mice and the influence of 1-deoxynojirimycin (DNJ) intervention on this effect. An obese mouse model was established by feeding a high-fat diet, and then DNJ at doses of 8.0, 4.0 and 2.0 mg/(kg mb·d) was administered to the obese mice for 45 days, and serum lipid levels and thyroid hormone related indicators were determined at 5 d intervals during this period. The results showed that compared with the obese control group, high-dose DNJ could significantly alleviate body mass gain and improve dyslipidemia caused by the high-fat diet in female and male mice, as well as increase total thyroxine (TT4) levels by 22.03% and 15.99% (P < 0.05), total triiodothyronine (TT3) levels by 66.86% and 47.79% (P < 0.05), decrease thyroid stimulating hormone (TSH) levels by 9.65% and 9.73% (P < 0.05), increase the activity of liver type 1 deiodinase (DIO1) by 40.99% and 24.40% (P < 0.05) and DIO1 protein levels by 35.68% and 29.50% (P < 0.05), and increase protein levels of liver thyroid hormone receptor β by 37.53% and 27.81% (P < 0.05) in female and male mice, respectively; and it could also significantly up-regulate the mRNA expression of liver DIO1, thyroid hormone receptor β, and the lipid metabolism-related thyroid hormone target genes encoding adipose triglyceride lipase (ATGL), carnitine palmitoyltransferase 1α (CPT1α) and cholesterol 7 alpha-hydroxylase (CYP7A1). In conclusion, DNJ can improve thyroid hormone homeostasis, up-regulate the mRNA expression of the lipid metabolism-related thyroid hormone target genes, and consequently promote lipid catabolism and inhibit body mass gain.

Key words: 1-deoxynojirimycin; thyroid hormone; obesity

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