食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 食品化学 •    下一篇

转谷氨酰胺酶-氯化镁协同诱导对冷榨豆粉凝胶的影响

王逢秋节1,杨鑫鑫1,谷雪莲1,栾滨羽2,3,黄雨洋1,朱颖1,朱秀清1   

  1. 1. 哈尔滨商业大学
    2. 黑龙江省哈尔滨市学海街1号哈尔滨商业大学食品工程学院
    3. 辽宁省沈阳市沈河区沈阳农业大学食品学院
  • 收稿日期:2021-08-06 修回日期:2022-05-05 出版日期:2022-08-25 发布日期:2022-08-19
  • 通讯作者: 朱秀清 E-mail:xqzhuwang@163.com

Effect of Synergistic Induction of Transglutaminase and Magnesium Chloride on Cold-pressed Soy Flour Gel

Feng-Qiu-Jie WangYang xinxin2,Xue-Lian N/AGU 2, Ying ZhuXiu-Qing ZHU   

  • Received:2021-08-06 Revised:2022-05-05 Online:2022-08-25 Published:2022-08-19
  • Contact: Xiu-Qing ZHU E-mail:xqzhuwang@163.com

摘要: 摘 要:冷榨豆粉是大豆低温榨油后的副产物,蛋白变性低,可以继续加工成豆制品。本研究以冷榨豆粉为原料,研究在转谷氨酰胺酶(transglutaminase,TGase) 与氯化镁(MgCl2)的协同诱导对其形成凝胶的影响。结果表明,以冷榨豆粉为原料制备的豆腐膳食纤维含量是卤水豆腐3倍,脂肪含量仅为卤水豆腐的1/3;TG酶的加入会明显改善冷榨豆粉凝胶内部水分,使其更为均匀,且制成的豆腐凝胶具有更高的储能模量G′(10498Pa),表明形成更牢固的凝胶网络结构,从而导致冷榨豆粉豆腐的持水性最大(51.01%)、出品率最高(244%);二级结构无规则卷曲含量最高(21.45%);维持凝胶网络的二硫键含量为(5.46%)接近卤水豆腐(7.02%);扫描电镜结果表明TG酶协同氯化镁使冷榨豆粉豆腐凝胶网状更为细致紧密,整体接近卤水豆腐。这表明,TG酶与氯化镁凝固剂协同作用可改善冷榨豆粉形成凝胶的品质,本研究为冷榨豆粉的多元开发提供了一定的理论基础。

关键词: 关键词:冷榨豆粉, 豆腐凝胶, 膳食纤维, 转谷氨酰胺酶, 流变学, 结构

Abstract: Abstract: Cold pressed soy flour is a by-product of soybean low-temperature oil pressing, with low protein denaturation and can be processed into soy products. In this study, cold-pressed soybean flour was used as raw material to study the effect of synergistic induction of transglutaminase (TGase) and magnesium chloride (MgCl2) on the formation of gel. The results show that the dietary fiber content of tofu prepared from cold-pressed soybean flour is three times that of brine tofu, and the fat content is only 1/3 of that of brine tofu; the addition of TG enzyme will significantly improve the internal moisture of the cold-pressed soybean flour gel, so that It is more uniform, and the prepared tofu gel has a higher storage modulus G′ (10498Pa), indicating that a stronger gel network structure is formed, which leads to the maximum water holding capacity of cold pressed soybean flour tofu (51.01%) ), the highest yield (244%); the highest content of random curls in the secondary structure (21.45%); the disulfide bond content that maintains the gel network is (5.46%) close to the brine tofu (7.02%); the scanning electron microscope results show that TG Enzymes and magnesium chloride make the cold-pressed soy flour and tofu gel network more detailed and compact, and the whole is close to brine tofu. This indicates that the synergistic effect of TG enzyme and magnesium chloride coagulant can improve the quality of cold-pressed soybean flour to form a gel. This study provides a certain theoretical basis for the multiple development of cold-pressed soybean flour.

Key words: Keywords: Cold pressed soybean meal, tofu gel, dietary fiber, transglutaminase, rheology, structure

中图分类号: