人乳与牛乳N-链寡糖组对小鼠肠道菌群的影响

doi:10.7506/spkx1002-6630-20210129-338

摘要/Abstract

摘要： 目的：探究人乳N-链寡糖组（human milk N-glycome，HMN）与牛乳N-链寡糖组（bovine milk N-glycome，BMN）对小鼠肠道菌群的影响。方法：以C57BL/6J雄性小鼠为对象，首先在体外测定粪便菌群中与N-链寡糖相关的糖苷酶活性，并模拟粪便菌群对HMN与BMN的利用；然后将小鼠随机分为对照组、HMN和BMN处理组，灌胃21 d后，测定盲肠内短链脂肪酸（short chain fatty acids，SCFAs）的含量，并采用16S rDNA高通量测序分析肠道菌群的变化。结果：鼠粪便菌群中能够检测到β-半乳糖甘酶、α-岩藻糖苷酶及β-N-乙酰氨基葡萄糖苷酶等活性，且HMN与BMN均可被粪便微生物降解利用；体内实验发现，与对照组相比，HMN的摄入显著增加了肠道中总SCFAs的含量（P＜0.05），而BMN组无显著变化（P＞0.05）；HMN与BMN的摄入均可显著影响鼠肠道菌群的组成，且两组之间也存在差异；在门水平上，与对照组相比，HMN的摄入降低了拟杆菌门的相对丰度，提高了厚壁菌门的相对丰度，而BMN的摄入降低了变形菌门的相对丰度；在属水平上，与对照组相比，摄入HMN与BMN后Muribaculaceae_norank与副鞭毛菌属的相对丰度均降低，拟杆菌属及粪肠球菌属的相对丰度均升高，HMN与BMN组之间的拟杆菌属、粪肠球菌属相对丰度差异不明显；此外，与对照组和BMN组相比，摄入HMN增加了小鼠肠道菌群中乳杆菌属、Erysipelotrichaceae-Unclassified与大肠埃希氏菌属的相对丰度，而BMN组的阿克曼菌属的相对丰度高于对照组与HMN处理组。结论：HMN与BMN能够显著影响小鼠肠道菌群的构成，实验可为了解N-链寡糖的功能和未来婴儿配方奶粉的开发提供参考。

关键词: 人乳N-链寡糖；牛乳N-链寡糖；糖苷酶；肠道微生物；短链脂肪酸

Abstract: In order to explore the effect of human milk N-glycome (HMN) and bovine milk N-glycome (BMN) on the intestinal flora in mice, glycosidase activity related to N-glycome in the fecal flora of C57BL/6J male mice was determined in vitro and the utilization of HMN and BMN by the fecal flora was simulated. The mice were randomly divided into control, HMN and BMN treatment groups. After 21 days of gavage, the contents of short chain fatty acids (SCFAs) in the cecum was detected, and 16S rDNA high-throughput sequencing was used to analyze the composition of intestinal microorganisms. The results showed that the activity of β-galactosidase, α-fucosidase and β-N-acetylglucosaminidase were detected in mouse fecal flora, and that HMN and BMN could be utilized by it. Compared with the control group, the intake of HMN increased the contents of total short-chain fatty acids (SCFAs) significantly (P < 0.05), while BMN did not (P > 0.05). The intake of HMN and BMN significantly changed the composition of intestinal microbes, a significant difference being observed between the two groups. At the phylum level, the intake of HMN reduced the relative abundance of Bacteroidetes and increased the relative abundance of Firmicutes, compared with the control group while the intake of BMN significantly reduced the relative abundance of Proteobacteria. And at the genus level, the relative abundance of Muribaculaceae_norank and Parasutterella were reduced in both HMN and BMN groups, whereas the relative abundance of Bacteroides and Enterococcus were increased compared with the control group. However, the relative abundance of Bacteroides and Enterococcus were not significantly different between the two groups. In addition, the relative abundance of Lactobacillus, Erysipelotrichaceae_Unclassified and Escherichia in the HMN group was higher than that in the control and BMN groups, while the relative abundance of Akkermansia in the BMN group was higher than that in the control and HMN groups. Generally, HMN and BMN could affect the structure and composition of mouse intestinal flora, which is important for understanding the function of N-glycome and applying it in the development of infant formula milk in the future.

Key words: human milk N-glycome; bovine milk N-glycome; glycosidase; gut microbiota; short chain fatty acids

中图分类号:

TS252.1

郭晶宇，陈亚然，毛慧敏，王婷，Josef VOGLMEIR，刘丽. 人乳与牛乳N-链寡糖组对小鼠肠道菌群的影响[J]. 食品科学, 2022, 43(1): 127-134.

GUO Jingyu, CHEN Yaran, MAO Huimin, WANG Ting, Josef VOGLMEIR, LIU Li. Effect of N-Glycome from Human Milk and Bovine Milk on Intestinal Microorganisms in Mice[J]. FOOD SCIENCE, 2022, 43(1): 127-134.

[1]	刘迪吕晓哲丛艳君李林峰梁萌高吉安邱学宇张倩倩. 特异性水解αs1-酪蛋白过敏原IgE作用表位aa83-105的蛋白酶筛选[J]. 食品科学, 0, (): 0-0.
[2]	郭晶宇陈亚然毛慧敏王婷 Josef Voglmeir 刘丽. 人乳与牛乳N-链寡糖组对小鼠肠道菌群的影响[J]. , 0, (): 0-0.
[3]	胡永芯谭宏凯胡巍熊子奕袁娟丽潘丽娜汪家琦李欣. 牛乳乳清中主要过敏原的B细胞表位定位[J]. 食品科学, 0, (): 0-0.
[4]	刘宇婷郭军王越男郭保民米智慧. SFC-Q-TOF-MS法鉴定四种家畜乳甘油三酯及特征分析[J]. , 0, (): 0-0.
[5]	简清梅，索化夷，张喜才，苟兴能，黄业传. 分子动力学模拟超高压结合热处理对β-乳球蛋白结构的影响[J]. 食品科学, 2021, 42(23): 57-63.
[6]	刘培玲，张晴晴，高增丽，杨岚，乌云，曹文慧，母智深. 乳清蛋白改性研究进展[J]. 食品科学, 2021, 42(23): 333-348.
[7]	王艺会蔡淼张敏赵华刘京杨贞耐. 酪蛋白水解肽对酸乳加工特性及生物活性的影响[J]. 食品科学, 0, (): 0-0.
[8]	柴玉霞王新宇岳喜庆杨梅. 驴乳外泌体中miRNA的测序与分析[J]. 食品科学, 0, (): 0-0.
[9]	关曼缇，张英春，卢卫红，王傲. 乳酸杆菌脂磷壁酸的结构及免疫调控功能研究进展[J]. 食品科学, 2021, 42(21): 278-284.
[10]	李晓东，潘悦，刘璐，朱启鹏. 乳脂肪球膜的特性、开发及在模拟母乳脂肪球结构中的应用[J]. 食品科学, 2021, 42(21): 372-379.
[11]	张丽娜瞿婧妍尹利昂刘君孙赵娜周鹏. 不同冻藏条件对人乳脂质及挥发性风味物质的影响[J]. 食品科学, 0, (): 0-0.
[12]	徐小爽韩翼宇李逍遥彭秋琦罗文静于景华. A1与A2 β-酪蛋白酸奶产品特性的比较[J]. 食品科学, 0, (): 0-0.
[13]	李晓东常淤浩潘悦林爽刘璐张宏达周星宇马欣. 不同脂肪来源婴儿配方奶粉与母乳在甘油脂组成上的差异分析[J]. , 0, (): 0-0.
[14]	杨续金，侯燕军，李立敏，呼斯乐，高爱武. 泌乳期间绵羊乳成分及酶凝乳特性变化规律[J]. 食品科学, 2021, 42(19): 129-133.
[15]	李晓东张更旭王宇鑫郝欣悦刘璐赵铭琪. 益生菌契达干酪抗氧化肽的结构及其体内代谢稳定性[J]. , 0, (): 0-0.

人乳与牛乳N-链寡糖组对小鼠肠道菌群的影响

Effect of N-Glycome from Human Milk and Bovine Milk on Intestinal Microorganisms in Mice

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价