食品科学 ›› 2022, Vol. 43 ›› Issue (17): 248-254.doi: 10.7506/spkx1002-6630-20210818-241

• 包装贮运 • 上一篇    下一篇

草莓脯贮藏期间非酶褐变的化学途径分析

牛丽影,胡丽丽,李大婧,刘春菊,张钟元,刘春泉,曹彬彬,顾千辉,谢婷婷,王云海   

  1. (1.江苏省农业科学院农产品加工研究所,江苏 南京 210014;2.三只松鼠股份有限公司,安徽 芜湖 241070)
  • 出版日期:2022-09-15 发布日期:2022-09-28
  • 基金资助:
    江苏省农业科技自主创新资金项目(CX(21)2026)

Chemical Pathway Analysis of Non-enzymatic Browning in Preserved Strawberry during Storage

NIU Liying, HU Lili, LI Dajing, LIU Chunju, ZHANG Zhongyuan, LIU Chunquan, CAO Binbin, GU Qianhui, XIE Tingting, WANG Yunhai   

  1. (1. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. Three Squirrel Companies Limited, Wuhu 241070, China)
  • Online:2022-09-15 Published:2022-09-28

摘要: 为确定贮藏期间草莓脯褐变的化学途径,将草莓脯分别于37、25、4 ℃条件下贮藏,测定其色泽以及抗坏血酸、总酚、3 种游离糖、5 种游离氨基酸和5-羟甲基糠醛(5-hydroxymethylfurfural,5-HMF)含量,并进行相关性和聚类分析。结果表明:随着贮藏时间延长,3 个温度下 草莓脯L*、a*、b*值均呈下降趋势,贮藏90 d色差ΔE为11.42~33.33,说明草莓脯贮藏期间色泽发生明显变化;抗坏血酸、总酚、游离糖和氨基酸含量出现不同程度的下降;5-HMF含量则呈线性上升;相关性分析结果显示除谷氨酰胺含量外,其他色泽及化学指标与ΔE呈显著、极显著或高度显著相关(P<0.05、P<0.01、P<0.001),说明色泽变化可能是抗坏血酸降解、多酚氧化和美拉德反应等途径的共同作用结果;聚类分析则将样品分为两类,较高温度、较长时间贮藏的样品与其他样品能够得以区分,说明温度与时间对褐变程度的影响不可忽略。总之,在草莓脯贮藏期间的褐变过程中,同时存在抗坏血酸、酚类、糖和氨基酸参与的褐变反应,但各成分含量变化与褐变程度存在不同步性。本实验结果可为草莓脯贮藏期间的褐变控制提供借鉴。

关键词: 草莓脯;贮藏;非酶褐变;理化指标;相关性分析

Abstract: In order to illustrate the chemical pathway of the browning of preserved strawberry during storage, commercial preserved strawberries were stored at 37, 25 or 4 ℃ and evaluated for color parameters and the contents of ascorbic acid, total phenol, three free sugars, five free amino acids and 5-hydroxymethyl furfural (5-HMF), and correlation and cluster analyses were performed as well. The results showed that for each temperature, the color parameters L*, a*, and b* of preserved strawberry all decreased with storage time, and the total color difference ΔE was 11.42–33.33 after 90 days of storage, suggesting that the color of preserved strawberry changed obviously during storage. Additionally, the contents of ascorbic acid, total phenol, free sugars, and free amino acids decreased in varying degrees, while 5-HMF content increased linearly. The correlation analysis showed that ΔE was significantly correlated with all color and chemical parameters except glutamine content (P < 0.05, P < 0.01, P < 0.001), indicating that the browning may result from a combination of ascorbic acid degradation, polyphenol oxidation and Maillard reaction. Using cluster analysis, the samples stored at higher temperature and for a longer period of time were distinguished from others, suggesting that the influence of storage temperature and period on the browning degree cannot be neglected. In conclusion, during storage, ascorbic acid, total phenol, free sugars and free amino acids are involved in the browning of preserved strawberry, despite the asynchrony. The results of this study provide a rationale for browning control of preserved strawberry.

Key words: preserved strawberry; storage; non-enzymatic browning; physicochemical parameters; correlation analysis

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