羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响

摘要/Abstract

摘要： 摘要：为果实软化一直是影响果实贮藏的关键性问题，严重影响了果实的经济价值。依据课题组前期研究结果，本研究以‘里约红’葡萄柚为研究试材，研究羧甲基壳聚糖（Carboxymethyl chitosan，CMCS）诱导培养罗伦隐球酵母（Cryptococcus laurentii，C. laurentii）处理对采后葡萄柚果实软化的控制效果。实验首先测定处理葡萄柚果实的硬度和失重情况，判断CMCS-C. laurentii处理对葡萄柚果实软化情况是否有影响。结果表明：CMCS-C. laurentii处理显著（P<0.05）抑制了葡萄柚果实的软化。在此基础上，对贮藏期间处理葡萄柚果实细胞壁多糖物质含量、多糖水解酶活性测定及果实细胞壁微观结构观察，结果表明：CMCS-C. laurentii处理能有效的维持果实内共价结合型果胶（SCSP）、紧密型半纤维素（24KSF）的含量，降低了果实中水溶性果胶（WSP）、离子型果胶（ESP）以及松散型半纤维素（4KSF）的含量；并且相较于对照组，处理组葡萄柚果实的相关酶活性（果胶甲酯酶（PE）、多聚半乳糖醛酸酶（PG）、纤维素酶（CX）、α-L-阿拉伯呋喃糖苷酶（α-L-Af）和β-葡萄糖苷酶（β-Gal））均得到显著抑制；细胞壁微观结构观察结果也表明，在贮藏中后期，处理后果实的细胞壁结构也更加稳定，胞内结果也相对完整。综上所述，CMCS-C. laurentii处理减缓采后葡萄柚果实细胞内部细胞器及细胞壁的降解，较好的维持细胞完整性，延缓果实软化。

关键词: 果实软化, 葡萄柚, 羧甲基壳聚糖, 罗伦隐球酵母, 细胞壁代谢

Abstract: Abstract: Softening of fruit is always a key problem affecting the storage of fruit, which seriously affects the economic value of fruit. Based on the previous research results, this study took 'Rio Red' grapefruit as the test material to study the control effect of Cryptococcus laurentii induced by Carboxymethyl chitosan (CMCS) on the softening of post-harvest grapefruit fruits. The experiment first determined the influence of CMCS-C. laurentii treatment on the softening of grapefruit fruit by measuring the hardness and weightlessness of grapefruit fruit before and after treatment. The results showed as follows: CMCS-C. laurentii treatment significantly (P<0.05) inhibited the softening of grapefruit fruit. Afterwards, the cell wall polysaccharide content, the polysaccharide hydrolase activity and the microstructure of the fruit cell wall of the treated grapefruits were measured during storage. The results showed that CMCS-C. laurentii treatment was effective in maintaining the content of covalently bound pectin (SCSP) and compact hemicellulose (24KSF) and reducing the content of water-soluble pectin (WSP), ionic pectin (ESP) and loose hemicellulose (4KSF) in the fruit; and compared to the control group, the related enzymes in the treated grapefruit fruit The cell wall microstructure also showed that the cell wall structure of the treated fruit was more stable and the intracellular results were relatively intact during the middle and late stages of storage. In conclusion, CMCS-C. laurentii treatment slowed down the degradation of organelles and cell walls in post-harvest grapefruit cells, better maintained cell integrity and delayed fruit softening.

Key words: Fruit softening, Citrus paradisi, Carboxymethyl chitosan, Cryptococcus laurentii, Cell wall metabolism

中图分类号:

杜嵘宇黄二宾吴化雨王芳邓佳. 羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响[J]. 食品科学.

FANG WANG. Effects of cell wall metabolism of grapefruit fruit induced by carboxymethyl chitosan induced cultured Cryptococcus laurentii treatment[J]. FOOD SCIENCE.

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