食品科学 ›› 2023, Vol. 44 ›› Issue (11): 152-159.doi: 10.7506/spkx1002-6630-20220623-260

• 包装贮运 • 上一篇    

外源果糖处理对采后杏果实软化的影响

芦玉佳,张昱,宋美玉,任新雅,张亚琳,张文娜,马海娟,朱璇   

  1. (新疆农业大学食品科学与药学学院,新疆 乌鲁木齐 830052)
  • 发布日期:2023-06-30
  • 基金资助:
    新疆维吾尔自治区自然科学基金资助项目(2022D01A196);南京农业大学-新疆农业大学联合基金项目(KYYJ201908); 新疆维吾尔自治区研究生科研创新项目(XJ2021G172); 2022年新疆农业大学自治区级大学生创新创业训练计划项目(S202210758087)

Effect of Exogenous Fructose Treatment on Postharvest Softening of Apricot Fruits

LU Yujia, ZHANG Yu, SONG Meiyu, REN Xinya, ZHANG Yalin, ZHANG Wenna, MA Haijuan, ZHU Xuan   

  1. (College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China)
  • Published:2023-06-30

摘要: 为研究果糖对杏果实软化的影响,本实验以‘赛买提’杏为试材,采用300 mmol/L的果糖溶液进行减压渗透处理(0.05 MPa、2 min),之后恢复常压浸泡5 min,以蒸馏水处理作为对照。处理后的杏果实贮藏于(0±1)℃冷库中。每7 d测定杏果实硬度、细胞膜渗透率和可溶性果胶(water soluble pectin,WSP)、螯合性果胶(chelate soluble pectin,CSP)、碳酸钠可溶性果胶(Na2CO3 soluble pectin,NSP)、纤维素含量及多聚半乳糖醛酸酶(polygalacturonase,PG)、果胶甲酯酶(pectin methyl esterase,PME)、β-葡萄糖苷酶(β-galactosidase,β-Gal)、纤维素酶(cellulase,CEL)活力,并使用透射电子显微镜观察杏果实的细胞超微结构。结果表明:与对照组相比,外源果糖处理能显著抑制杏果实细胞壁降解酶的活性,较好地维持CSP、NSP和纤维素含量,显著抑制细胞壁的降解。在贮藏49 d时,与对照组相比,果糖处理组杏果实CSP、NSP和纤维素含量分别比对照组高31.30%、10.58%、14.30%(P<0.05);对照组WSP含量和细胞膜渗透率分别是果糖处理组的1.09、1.25 倍;外源果糖处理还能显著维持杏果实细胞器和膜系统的完整性,有效延缓杏果实硬度的下降。

关键词: 杏果实;果糖;细胞壁代谢;软化?

Abstract: The present research was undertaken in order to study the effect of exogenous fructose treatment on postharvest softening of apricot fruits. ‘Saimaiti’ apricots were subjected to osmotic treatment in 300 mmol/L fructose solution at 0.05 MPa for 2 min followed by normal pressure for another 5 min before storage at (0 ± 1) ℃. Distilled water was used as control treatment. Fruit hardness, cell membrane permeability, water soluble pectin (WSP), chelate soluble pectin (CSP), Na2CO3 soluble pectin (NSP) and cellulose contents, and polygalacturonase (PG), pectin methyl esterase (PME), β-galactosidase (β-Gal) and cellulase (CEL) activities were determined every seven days during storage, and cell ultrastructure was observed by transmission electron microscopy (TEM). The results showed that exogenous fructose treatment could significantly inhibit the activity of cell wall degrading enzymes and maintain the contents of CSP, NSP, and cellulose, thereby inhibiting the degradation of the cell wall. On day 49 of storage, compared with the control group, the contents of CSP, NSP and cellulose in fructose-treated apricot fruits increased by 31.30%, 10.58%, and 14.30% (P < 0.05). The WSP content and cell membrane permeability of the control group were 1.09 and 1.25 times as high as those in the control group, respectively. Furthermore, this treatment maintained the integrity of organelles and the membrane system, and effectively delayed the decline in fruit hardness.

Key words: apricot fruit; fructose; cell wall metabolism; softening

中图分类号: