食品科学 ›› 2023, Vol. 44 ›› Issue (9): 72-81.doi: 10.7506/spkx1002-6630-20220620-204

• 营养卫生 • 上一篇    下一篇

原儿茶酸通过Toll样受体4/核因子κB途径减轻高脂饮食诱导的肝脏炎症

焦小文,李佳,李云龙,韩林,王敏   

  1. (1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;2.山西农业大学山西功能食品研究所,山西 太原 030031)
  • 出版日期:2023-05-15 发布日期:2023-05-24
  • 基金资助:
    国家自然科学基金青年科学基金项目(32001701);国家现代农业产业技术体系建设专项(CARS-08-E2-1)

Intake of Protocatechuic Acid Attenuates High-Fat Diet Induced Liver Inflammation via the Toll Like Receptor 4/Nuclear Factor κB Pathway

JIAO Xiaowen, LI Jia, LI Yunlong, HAN Lin, WANG Min   

  1. (1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;2. Institute of Functional Food of Shanxi, Shanxi Agricultural University, Taiyuan 030031, China)
  • Online:2023-05-15 Published:2023-05-24

摘要: 原儿茶酸(protocatechuic acid,PCA)是花青素在体内的主要代谢产物之一,具有良好的抗炎活性。本实验利用高脂饮食(high fat diet,HFD)诱导C57BL/6J小鼠肝脏炎症,通过灌胃100 mg/(kg mb·d)PCA研究其对小鼠肝脏的保护效果,并通过体外实验进一步研究其潜在机制。体内研究结果表明,PCA干预12 周后,可显著降低HFD诱导C57BL/6J小鼠的体质量和肝脏脂肪含量;血清和肝脏中白细胞介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子α(tumor necrosis factor α,TNF-α)和脂多糖(lipopolysaccharide,LPS)等炎症因子水平均明显降低。体外转录组测序及原代肝细胞和肝脏的免疫印迹分析结果表明,PCA可显著降低Toll样受体4(Toll like receptor 4,TLR4)基因和蛋白的表达,然后通过下调核因子κB(nuclear factor κB,NF-κB)的磷酸化抑制炎症因子(如IL-6)的表达。综上,PCA可有效改善HFD诱导的肝脏炎症,其可能是通过下调TLR4/NF-κB信号通路来实现。

关键词: 原儿茶酸;肝脏炎症;转录组测序;Toll样受体4;核因子κB

Abstract: Protocatechuic acid (PCA) is one of the main metabolites of anthocyanins in the body and has good anti-inflammatory activity. In this study, the protective effect of oral administration of PCA at a dose of 100 mg/(kg mb·d) on high-fat diet (HFD)-induced liver inflammation in C57BL/6J mice was investigated, and the underlying mechanism was investigated by in vitro experiments. In vivo studies showed that after 12 weeks of PCA intervention, the body mass and liver fat content of HFD-fed C57BL/6J mice were significantly reduced, and so were the levels of inflammatory factors such as interleukin (IL)-1β, IL-6, tumor necrosis factor α (TNF-α) and lipopolysaccharide (LPS) in serum and liver. In vitro transcriptome sequencing (RNA-Seq) and Western blot analysis of primary hepatocytes and livers demonstrated that PCA could significantly reduce the gene and protein expression of Toll like receptor 4 (TLR4), and then down-regulate the phosphorylation of nuclear transcription factor κB (NF-κB) inhibiting the expression of inflammatory factors such as IL-6. These results suggest that PCA can effectively improve HFD-induced liver inflammation, possibly by down-regulating the TLR4/NF-κB signaling pathway.

Key words: protocatechuic acid; hepatic inflammation; RNA sequencing; Toll like receptor 4; nuclear factor κB

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