平卧菊三七提取物降尿酸活性的物质基础分析

摘要/Abstract

摘要： 为探究平卧菊三七降尿酸活性物质基础，评价了不同乙醇浓度（0%、30%、70%）回流提取对平卧菊三七提取物有效成分含量、黄嘌呤氧化酶(xanthine oxidase，XOD)抑制能力及抗氧化活性的影响，利用非靶标代谢组学技术对提取物进行物质鉴定分析，同时，建立次黄嘌呤和氧嗪酸钾造模的高尿酸血症小鼠模型，以抑酶活性较高的30%醇提物和70%醇提物进行体内降尿酸效果评价。结果表明，提取物的抑酶能力和总黄酮、总有机酸含量呈正相关（p<0.05），超氧阴离子清除能力与总酚含量呈正相关（p<0.05）。非靶向代谢组学共检测出705种差异代谢物，结合体外实验结果推测柚皮素、1,5-二咖啡酰奎宁酸、α-亚麻酸、阿魏酸、香叶木素等物质为平卧菊三七降尿酸关键物质。30%和70%醇提物均可通过降低血清尿酸和抑制XOD来缓解高尿酸血症（p<0.01），且一定程度上缓解高尿酸血症带来的肝脏氧化损伤。本研究通过体内和体外实验验证平卧菊三七具备降尿酸活性，对平卧菊三七降尿酸物质基础进行探讨，为平卧菊三七降尿酸活性物质的制备和功能食品开发提供理论依据。

关键词: 平卧菊三七, 高尿酸血症, 非靶向代谢组学, 黄嘌呤氧化酶, 尿酸

Abstract: In order to investigate the material basis of hypouricemic activity of Gynura procumbens, the effects of reflux extraction with different ethanol concentrations (0%, 30% and 70%) on the content of active ingredients, xanthine oxidase (XOD) inhibitory ability and antioxidant activity of Gynura procumbens extract were evaluated. The extracts were analyzed and identified by non-targeted metabolism techniques. Meanwhile, the hyperuricemia mouse model was established by hypoxanthine and potassium oxonate, and the 30% and 70% alcoholic extracts with higher enzyme inhibitory activity were used to evaluate the effect of reducing uric acid in vivo.The results showed that the enzyme inhibitory capacity of the extract was positively correlated with the total flavonoid and total organic acid contents (p<0.05), and the superoxide anion scavenging capacity was positively correlated with the total phenol content (p<0.05). A total of 705 differential metabolites were detected by non-targeted metabolomics. Combined with the results of in vitro experiments, naringenin, 1,5-dicaffeoylquinic acid, α-linolenic acid, ferulic acid, diosmetin and other substances were hypothesized to be the key substances.for lowering uric acid in Gynura procumbens. Both 30% and 70% alcohol extracts alleviated hyperuricemia by lowering serum uric acid and inhibiting XOD (p<0.01), and at the same time alleviate the liver oxidative damage caused by hyperuricemia to a certain extent. In this study, the hypouricemic activity of Gynura procumbens was verified by in vivo and in vitro experiments. The material basis of hypouricemic activity of Gynura procumbens was discussed, which provided a theoretical basis for the preparation of hypouricemic active substances and the development of functional foods.

Key words: Gynura procumbens, hyperuricemia, non-targeted metabolomics, xanthine oxidase, uric acid

中图分类号:

TS202.1

马文婧付桂明赵富强林素钦万茵. 平卧菊三七提取物降尿酸活性的物质基础分析[J]. 食品科学.

GUIMING FU YIN WAN. Analysis of uric acid-lowering activity and material basis of Gynura procumbens extract[J]. FOOD SCIENCE.

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