余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用

doi:10.7506/spkx1002-6630-20220913-112

食品科学 ›› 2023, Vol. 44 ›› Issue (15): 156-171.doi: 10.7506/spkx1002-6630-20220913-112

• 营养卫生 • 上一篇

余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用

李瑞婷，何维，宋贺，谢小亭，吴杰，姜燕

（1.大理大学公共卫生学院，云南大理 671000；2.川北医学院，四川南充 637000；3.川北医学院第二附属医院口腔科，四川南充 637100；4.宾川佳泓园艺有限责任公司，云南宾川 671600）

发布日期:2023-09-01
基金资助:
大理大学博士启动基金项目（KTBS2018005）；云南省大学生创新创业训练计划项目（202110679027）；云南省教育厅科学研究基金项目（2022Y807）；云南省乡村振兴产业关键技术集成示范项目（202204BP090017）

Protective Effect of Freeze-Dried Fruit Powder of Phyllanthus emblica L. on Acute and Chronic Hyperuricemia and Liver and Kidney Injuries in Mice

LI Ruiting, HE Wei, SONG He, XIE Xiaoting, WU Jie, JIANG Yan

(1. School of Public Health, Dali University, Dali 671000, China; 2. North Sichuan Medical College, Nanchong 637000, China; 3. Department of Stomatology, The Second Affiliated Hospital of North Sichuan Medical College, Nanchong 637100, China; 4. Binchuan Jiahong Horticulture Co., Ltd., Binchuan 671600, China)

Published:2023-09-01

摘要/Abstract

摘要： 目的：探究余甘子冻干粉对急性和慢性高尿酸血症（hyperuricemia，HUA）小鼠的降尿酸（uric acid，UA）效果及对肝肾的保护作用。方法：分别采用腹腔注射次黄嘌呤和氧嗪酸钾建立急性和慢性HUA小鼠模型，灌胃75、150、300 mg/kg mb余甘子冻干粉进行干预，连续7 d，检测小鼠血清UA、血清尿素氮（blood urea nitrogen，BUN）、肌酐（creatinine，CRE）水平和天冬氨酸氨基转移酶（aspartate aminotransferase，AST）、谷氨酸氨基转移酶（alanine aminotransferase，ALT）以及血清和肝脏组织中的黄嘌呤氧化酶（xanthine oxidase，XOD）和腺苷脱氨酶（adenosine deaminase，ADA）活力，计算肝/肾系数，苏木精-伊红染色法观察肝肾组织的病理学特征。结果：与模型组相比，150、300 mg/kg mb的余甘子冻干粉可极显著降低急性HUA小鼠肝脏系数和肾脏系数（P＜0.01），显著或极显著下调血清UA、BUN及CRE和AST水平（P＜0.05、P＜0.01）；75、150、300 mg/kg mb余甘子冻干粉可极显著抑制血清和肝脏中XOD和ADA活力（P＜0.01），300 mg/kg mb的余甘子冻干粉可极显著下调血清ALT水平（P＜0.01）。75、150、300 mg/kg mb余甘子冻干粉能极显著降低慢性HUA小鼠肝脏系数和肾脏系数（P＜0.01），极显著下调血清UA、BUN和ALT水平（P＜0.01），极显著抑制血清和肝脏中XOD以及血清中ADA活力（P＜0.01）；150、300 mg/kg mb的余甘子冻干粉可极显著下调血清CRE、AST水平以及肝脏中ADA活力（P＜0.01）。病理学分析结果提示，余甘子冻干粉可改善急性和慢性HUA小鼠肝脏和肾脏的病理变化。结论：余甘子冻干粉能有效降低急性和慢性HUA小鼠的血清UA水平，可能与抑制血清和肝脏中的XOD和ADA活力及促进肾脏UA排泄有关，同时发挥了一定的肾脏和肝脏保护作用。

关键词: 余甘子；高尿酸血症；黄嘌呤氧化酶；腺苷脱氨酶；肝肾保护

Abstract: Objective: To explore the protective effect of the freeze-dried fruit powder of Phyllanthus emblica L. (PEFP) against acute and chronic hyperuricemia (HUA) and liver and kidney injuries in mice. Methods: Mouse models of acute and chronic HUA were established by intraperitoneal injection of hypoxanthine and potassium oxazinate, respectively. Meanwhile, PEFP at 75, 150 and 300 mg/kg mb were orally given to the mice in the intervention group for seven days. The levels of uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum, and the activities of xanthine oxidase (XOD) and adenosine deaminase (ADA) in the serum and liver were detected. Liver and kidney indexes were calculated, and the pathological characteristics of liver and kidney tissues were observed by hematoxylin and eosin (HE) staining. Results: Compared with the model group, PEFP at 150 and 300 mg/kg mb significantly reduced the liver and kidney indexes (P < 0.01) and lowered serum UA, BUN, CRE, and AST levels in mice with acute hyperuricemia (P < 0.05 or P < 0.01). PEFP at 75, 150 and 300 mg/kg mb significantly inhibited serum and liver XOD and ADA activities (P < 0.01), and PEFP at 300 mg/kg mb lowered serum ALT levels in mice with acute hyperuricemia (P < 0.01). Meanwhile, all three doses of PEFP significantly reduced the liver and kidney indexes (P < 0.01), lowered serum UA, BUN and ALT levels (P < 0.01), and inhibited serum and liver XOD activities and serum ADA activity in mice with chronic hyperuricemia (P < 0.01). PEFP at 150 and 300 mg/kg mb lowered serum CRE and AST levels and liver ADA activity (P < 0.01). PEFP ameliorated the pathological changes of the liver and kidney in mice with acute and chronic hyperuricemia. Conclusion: PEPP can effectively decrease serum UA levels in mice with acute and chronic hyperuricemia, which may be related to the inhibition of XOD and ADA activities in the serum and liver, and the promotion of renal uric acid excretion, and can also exert renal and hepatoprotective effects.

Key words: Phyllanthus emblica L.; hyperuricemia; xanthine oxidase; adenosine deaminase; hepatorenal protection

中图分类号:

TS201.4

李瑞婷，何维，宋贺，谢小亭，吴杰，姜燕. 余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用[J]. 食品科学, 2023, 44(15): 156-171.

LI Ruiting, HE Wei, SONG He, XIE Xiaoting, WU Jie, JIANG Yan. Protective Effect of Freeze-Dried Fruit Powder of Phyllanthus emblica L. on Acute and Chronic Hyperuricemia and Liver and Kidney Injuries in Mice[J]. FOOD SCIENCE, 2023, 44(15): 156-171.

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余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用

Protective Effect of Freeze-Dried Fruit Powder of Phyllanthus emblica L. on Acute and Chronic Hyperuricemia and Liver and Kidney Injuries in Mice

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