芜菁酸性多糖结构及对脂多糖诱导肺损伤小鼠的保护作用

doi:10.7506/spkx1002-6630-20221104-046

食品科学 ›› 2023, Vol. 44 ›› Issue (13): 52-59.doi: 10.7506/spkx1002-6630-20221104-046

芜菁酸性多糖结构及对脂多糖诱导肺损伤小鼠的保护作用

卡地尔亚·库尔班，阿吉然姆·阿布拉，陈卓尔，米合热尼沙·阿木热江，海力茜·陶尔大洪，杨飞

（1.新疆医科大学药学院，新疆乌鲁木齐 830011；2.新疆维吾尔自治区分析测试研究院，新疆乌鲁木齐 830011）

出版日期:2023-07-15 发布日期:2023-08-11
基金资助:
国家自然科学基金地区科学基金项目（81960765）

Structure of an Acidic Polysaccharide from Brassica rapa L. and Its Protective Effect on Lipopolysaccharide-Induced Lung Injury in Mice

Kadierya·KUERBAN, Ajiranmu·Abula, CHEN Zhuo’er, Miherenisha·AMUREJIANG, Hailiqian·TAOERDAHONG, YANG Fei

(1. School of Pharmacy, Xinjiang Medical University, ürümqi 830011, China;2. Xinjiang Uygur Autonomous Region Research Institute of Analysis and Testing, ürümqi 830011, China)

Online:2023-07-15 Published:2023-08-11

摘要/Abstract

摘要： 目的：研究芜菁酸性多糖（Brassica rapa L. acidic polysachharide-1，BRAP-1）的结构及其对脂多糖（lipopolysaccharide，LPS）诱导的小鼠肺损伤的影响及其机制。方法：采用苯酚硫酸法、间羟基联苯法、考马斯亮蓝法、高效凝胶渗透色谱、傅里叶变换红外光谱、气相色谱-质谱法对BRAP-1化学组成、分子质量、结构及单糖组成进行分析。动物实验正常组、模型组小鼠灌胃0.5 mL/d生理盐水，BRAP-1低、中、高剂量组分别灌胃50、100、200 mg/（kg mb·d），持续10 d，采用LPS气管滴注法建立肺损伤模型，检测相关指标，包括肺湿质量/干质量比值、肺泡灌洗液蛋白质量浓度、总细胞数，并进行苏木精-伊红染色；采用酶联免疫吸附测试法测定各组小鼠血清肿瘤坏死因子-α、白细胞介素-6（interleukin-6，IL-6）、IL-10、干扰素γ水平；采用实时荧光定量聚合酶链式反应法检测肺组织中肿瘤坏死因子受体、Toll样受体4、核转录因子-κB p65（nuclear factor kappa B p65，NF-κB p65）、IL-1β、NOD样受体3（NOD-like receptor 3，NLRP3）基因mRNA表达水平。结果：与模型组相比，BRAP-1对肺损伤小鼠肺组织水肿、肺泡浸润、蛋白渗出、细胞因子过度释放等病理学改变有缓解作用；并显著降低NF-κB/NLRP3通路关键基因mRNA表达水平。结论：BRAP-1对LPS诱导的小鼠肺损伤具有显著保护作用，其机制可能是调节NF-κB/NLRP3信号通路。

关键词: 芜菁酸性多糖；肺损伤；保护作用；NF-κB/NLRP3信号通路

Abstract: Objective: To study the structure of BRAP-1, an acidic polysaccharide from Brassica rapa L. and its effect and mechanism on lipopolysaccharide (LPS)-induced lung injury in mice. Methods: The chemical composition, molecular mass, structure and monosaccharide composition were analyzed by the phenol-sulfuric acid method, the m-hydroxydiphenyl method, the Coomassie brilliant blue method, high performance gel permeation chromatography (HPGPC), Fourier transform infrared (FT-IR) spectroscopy and gas chromatography-mass chromatography (GC-MS). The mice in the control and model groups were gavaged with 0.5 mL/d of phosphate buffered saline (PBS) and those in the BRAP-1 groups 50, 100 and 200 mg/(kg mb·d) of BRAP-1 for 10 days, respectively. A lung injury model was established by intratracheal LPS infusion. Lung wet/dry mass ratio, broncho-alveolar lavage fluid (BALF) protein concentration and total cell count were measured and hematoxylin-eosin (HE) staining was performed to evaluate histopathological changes. An enzyme-linked immunosorbent assay (ELISA) was used to determine the contents of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10 and interferon-γ (IFN-γ) in the serum of mice in each group. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the mRNA expression of tumor necrosis factor receptor (TNFR), Toll-like receptor 4 (TLR4), nuclear factor kappa B p65 (NF-κB p65), IL-1β and NOD-like receptor 3 (NLRP3) in lung tissue. Results: Compared with the model group, BRAP-1 alleviated the pathological changes of lung tissue such as edema, alveolar infiltration, protein exudation and excessive cytokines release in mice with lung injury, and significantly decreased the mRNA expression of key genes in the NF-κB/NLRP3 signaling pathway. Conclusion: BRAP-1 has a significantly protective effect on LPS-induced lung injury in mice, and the mechanism may be associated with the regulation of the NF-κB/NLRP3 signaling pathway.

Key words: Brassica rapa L. acidic polysaccharide; lung injury; protective effect; NF-κB/NLRP3 pathway

中图分类号:

TS201.4

卡地尔亚·库尔班，阿吉然姆·阿布拉，陈卓尔，米合热尼沙·阿木热江，海力茜·陶尔大洪，杨飞. 芜菁酸性多糖结构及对脂多糖诱导肺损伤小鼠的保护作用[J]. 食品科学, 2023, 44(13): 52-59.

Kadierya·KUERBAN, Ajiranmu·Abula, CHEN Zhuo’er, Miherenisha·AMUREJIANG, Hailiqian·TAOERDAHONG, YANG Fei. Structure of an Acidic Polysaccharide from Brassica rapa L. and Its Protective Effect on Lipopolysaccharide-Induced Lung Injury in Mice[J]. FOOD SCIENCE, 2023, 44(13): 52-59.

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芜菁酸性多糖结构及对脂多糖诱导肺损伤小鼠的保护作用

Structure of an Acidic Polysaccharide from Brassica rapa L. and Its Protective Effect on Lipopolysaccharide-Induced Lung Injury in Mice

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