穇子总黄酮成分及对秀丽隐杆线虫抗衰老活性分析

摘要/Abstract

摘要： 传统杂粮穇子（Eleusine coracana (L.) Gaertn.）营养丰富，具有一定保健功能，深受地方民众的喜爱。本文对其总黄酮进行提取和成分分析，并以秀丽隐杆线虫为模型研究总黄酮的抗衰老活性，为穇子的功能性应用提供理论依据。70%乙醇提取、乙酸乙酯萃取和D101大孔树脂纯化得到穇子总黄酮样品（Eleusine coracana flavonoids，ECFs），采用NaNO2-Al(NO3)3-NaOH分光光度法测定总黄酮含量，UPLC-MS/MS进行成分分析，化学法测定体外抗氧化活性，最后以秀丽隐杆线虫为模型系统评价ECFs的抗衰老活性。含量及成分分析结果显示，ECFs样品中总黄酮占82.6%，含量较高的成分为16个，其中以儿茶素相对含量最高。体外抗氧化结果显示，ECFs对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基和2,2-氨基-二(3-乙基-苯并噻唑啉-6-磺酸)（2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid，ABTS）自由基的清除能力的半抑制质量浓度（50%inhibitingconcentration，IC50）分别为45.34μg/mL和22.75μg/mL，其中对ABTS自由基的IC50甚至优于阳性对照Ve（29.25μg/mL）。在实验浓度下，与对照组相比，线虫内过氧化氢酶活力提高了53.98%，超氧化物歧化酶的活力提高30.64%，谷胱甘肽水平提高51.06%，丙二醛的含量降低了12.53%；ECFs延长线虫最长寿命17.86%；ECFs组的线虫移动力均优于对照组，表明ECFs提升了线虫的生命活力；对线虫产卵量无显著影响，表明样品对线虫生殖功能无促进作用；直接反映衰老程度的脂褐素沉积减少了53.91%。上述结果表明ECFs具有显著的体内外抗氧化效果，和显著的线虫抗衰老活性，这为穇子米的功能性应用提供了基础。

关键词: 穇子, 总黄酮, 秀丽隐杆线虫, 抗衰老活性

Abstract: Traditional finger millet (Eleusine coracana (L.) Gaertn.) is rich in nutrients and possesses certain health benefits, making it highly popular among local communities. In this study, we extracted and analyzed the total flavonoids from finger millet and investigated their anti-aging activity using the model organism Caenorhabditis elegans, providing a theoretical basis for the functional application of finger millet. Finger millet total flavonoid samples (Eleusine coracana flavonoids, ECFs) were obtained through 70% ethanol extraction, ethyl acetate extraction, and purification with D101 macroporous resin. The total flavonoid content was determined using the NaNO2-Al(NO3)3-NaOH spectrophotometric method, and component analysis was conducted using UPLC-MS/MS. In vitro antioxidant activity was evaluated using chemical assays, and the anti-aging activity of ECFs was assessed using C. elegans as a model organism. The results of content and component analysis revealed that total flavonoids accounted for 82.6% of ECFs, with 16 major components identified, among which epicatechin had the highest relative content. In vitro antioxidant results showed that ECFs exhibited half-maximal inhibitory concentrations (IC50) of 45.34 μg/mL for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 22.75 μg/mL for 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid (ABTS) radicals), with the IC50 for ABTS radicals even superior to the positive control vitamin E (29.25 μg/mL). Under experimental concentrations, compared to the control group, the activity of catalase increased by 53.98%, superoxide dismutase activity increased by 30.64%, glutathione levels increased by 51.06%, and malondialdehyde content decreased by 12.53% in C. elegans treated with ECFs. ECFs extended the lifespan of C. elegans by 17.86%, and their mobility was better than that of the control group, indicating improved vitality. ECFs had no significant effect on C. elegans reproduction, suggesting no promotional effect on reproductive function. Additionally, lipofuscin deposition, a direct indicator of aging, decreased by 53.91%. These results demonstrate the significant in vitro and in vivo antioxidant effects of ECFs, as well as remarkable anti-aging activity in C. elegans, providing a foundation for the functional application of finger millet.

Key words: Eleusine coracana (L.) Gaertn., total flavonoids, Caenorhabditis elegans, anti-aging

中图分类号:

TS201.4

王赛男陈丹妮胡克坚刘嘉敏李康瑗王嘉炜陈立平王祺皓肖苏尧. 穇子总黄酮成分及对秀丽隐杆线虫抗衰老活性分析[J]. 食品科学.

Sai-Nan Wang Dan CHEN Su-Yao XIAO. Analysis of Total Flavone Components of Finger Millet (Eleusine coracana (L.) Gaertn.) and Study on Anti-aging Activity of Caenorhabditis elegans[J]. FOOD SCIENCE.

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