基于Ag NPs/MXenes和CRISPR/Cas12a的ECL传感器构建及黄曲霉毒素B1检测应用

摘要/Abstract

摘要： 黄曲霉毒素B1（Aflatoxin B1, AFB1）是黄曲霉毒素家族中毒性最强的真菌毒素，痕量残留即对食品安全与人体健康构成严重威胁。本研究通过在MXenes纳米片上锚定银纳米颗粒（Ag NPs），成功制备了Ag NPs/MXenes复合材料，并将其作为鲁米诺-过氧化氢（Luminol-H2O2）电化学发光体系中的共反应促进剂，显著增强了ECL信号的稳定性和强度。以此材料为传感基底，结合CRISPR/Cas12a系统，构建了一种基于电化学发光共振能量转移（Electrochemiluminescence Resonance Energy Transfer，ECL-RET）的“关-开”型AFB1传感器。在目标物AFB1存在条件下，其可特异性识别并结合适配体，释放激活链，从而启动CRISPR/Cas12a反式切割活性，通过适配体调控的Cas12a激活过程，实现了对AFB1的高灵敏定量分析。该传感器在1.0 pg/mL-100 ng/mL浓度范围内呈现良好线性关系，检出限为0.4 pg/mL。实际样品检测结果表明该方法具有良好的适用性，为AFB1污染的早期监控提供了一种高效的即时检测技术。

关键词: Ag NPs/MXenes, 黄曲霉毒素B1, 电致化学发光传感器, 快速检测, CRISPR/Cas12a

Abstract: Aflatoxin B1 (AFB1), the most toxic member of the aflatoxin family, poses a serious threat to food safety and human health even at trace levels. In this study, a composite material designated Ag NPs/MXenes was successfully prepared by anchoring silver nanoparticles (Ag NPs) onto MXene nanosheets. This material served as an efficient co reaction accelerator in the Luminol H2O2 electrochemiluminescence (ECL) system, significantly enhancing both the stability and intensity of the ECL signal. Using Ag NPs/MXenes as the sensing substrate and integrating it with the CRISPR/Cas12a system, an ECL resonance energy transfer (ECL RET) based "off on" switch type biosensor was constructed for the detection of AFB1. In the presence of the target AFB1, the aptamer specifically binds to AFB1, resulting in the release of an activator strand that triggers the trans cleavage activity of CRISPR/Cas12a. Through this aptamer regulated activation of Cas12a, highly sensitive quantification of AFB1 was achieved. The sensor exhibited a good linear response over the concentration range of 1.0?pg/mL to 100?ng/mL, with a detection limit as low as 0.4?pg/mL. Practical application in real samples demonstrated satisfactory applicability, offering an efficient point of care testing (POCT) strategy for the early monitoring of AFB1 contamination.

Key words: Ag NPs/MXenes, AFB1, Electrochemiluminescence sensor, Rapid detection, CRISPR/Cas12a

中图分类号:

TS207.5

龙泽坤李玥宋雨默张晓波曹际娟. 基于Ag NPs/MXenes和CRISPR/Cas12a的ECL传感器构建及黄曲霉毒素B1检测应用[J]. 食品科学.

Ze-Kun Ji-Juan CAO. Construction of an ECL Sensor Based on Ag NPs/MXenes and CRISPR/Cas12a and Its Application in Aflatoxin B1 Detection[J]. FOOD SCIENCE.

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