食品科学 ›› 2010, Vol. 31 ›› Issue (24): 334-336.doi: 10.7506/spkx1002-6630-201024073

• 分析检测 • 上一篇    下一篇

蓝莓类饮料中花青素的快速检测方法

宋阳成1,陈玉娟2,李 皓1,陈小桦3,陈长武1,任保国   

  1. 1. 吉林工程技术师范学院
    2. 长春理工大学
    3. 黔南民族职业技术学院农学院
  • 收稿日期:2010-09-16 修回日期:2010-11-15 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 宋阳成 E-mail:songyangchengsych@163.com

Rapid Detection Methods for Anthocyanins in Blueberry Beverage

SONG Yang-cheng1,CHEN Yu-juan2,LI Hao1,CHEN Xiao-hua3,CHEN Chang-wu1,REN Bao-guo1   

  1. 1. College of Food, Jilin Teachers Institute of Engineering and Technology, Changchun 130052, China;
    2. College of Life Science, Changchun University of Science and Technology, Changchun 130000, China;
    3.College of Agronomy, Qiannan Vocational and Technical College for Nationalities, Xingyi 562400, China
  • Received:2010-09-16 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
  • Contact: SONG Yang-cheng E-mail:songyangchengsych@163.com

摘要:

目的:建立蓝莓饮料中飞燕草定-3-O- 葡萄糖苷及锦葵定-3-O- 葡萄糖苷两种花青素类成分的高效液相色谱快速检测方法。方法:采用高效液相色谱法,使用安捷伦ZORBAX SB-C18(4.6mm × 75mm,3.5μm)色谱柱,以3% 磷酸溶液和甲醇为流动相,二极管阵列检测器在波长525nm 处进行检测。结果:该方法对飞燕草定-3-O- 葡萄糖苷和锦葵定-3-O- 葡萄糖苷的最低检出限分别为0.4ng 和2.5ng,标准曲线线性良好,相关系数大于0.9984,回收率在90.2%~98.3% 之间,相对标准偏差不大于1.79%。结论:该法简洁、快速且灵敏度高,适合于蓝莓类饮料中花青素的快速检测。

关键词: 蓝莓, 饮料, 花青素, 高效液相色谱法

Abstract:

Objective: To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of delphinidin-3-O-glucoside and malvidin-3-O-glucoside in blueberry beverage. Methods: The anthocyanins including delphinidin-3-O-glucoside and malvidin-3-O-glucoside were determined by HPLC with ZORBAX SB-C18 (4.6 mm × 75 mm, 3.5μm) as the chromatographic column, 3% phosphoric acid-methanol as the mobile phase, diode array as the detector and detection wavelength at 525 nm. Results: The detection limits of this HPLC method for delphinidin-3-O-glucoside and malvidin-3-Oglucoside were 0.4 ng and 2.5 ng, respectively. The standard curve determined by this method exhibited an excellent linear relationship with correlation coefficient larger than 0.9984. The recovery rate of this method was in the range of 90.2%-98.3% with relative standard deviation less than 1.79%. Conclusion: The established HPLC method is a simple, rapid and sensitive determination method, which is suitable for the rapid determination of anthocyanins in blueberry beverage.

Key words: beverage, blueberry, Anthocyanins, HPLC

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