食品科学 ›› 2010, Vol. 31 ›› Issue (22): 237-241.doi: 10.7506/spkx1002-6630-201022050

• 工艺技术 • 上一篇    下一篇

酶解斑点叉尾鮰内脏制备血管紧张素转换酶抑制产物

杨晓军,陆剑锋* ,林 琳,段瑞霞,翁世兵,叶应旺,姜绍通   

  1. 合肥工业大学生物与食品工程学院
  • 收稿日期:2010-06-30 修回日期:2010-11-09 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 陆剑锋 E-mail:lujf@sibs.ac.cn
  • 基金资助:

    安徽省“十一五”重大科技攻关专项(08010301078);科技部“星火计划”项目(2008GA710021)

Enzymatic Preparation of Angiotensin Ⅰ -converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish

YANG Xiao-jun,LU Jian-feng*,LIN Lin,DUAN Rui-xia,WENG Shi-bing,YE Ying-wang,JIANG Shao-tong   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Received:2010-06-30 Revised:2010-11-09 Online:2010-11-25 Published:2010-12-29
  • Contact: LU Jian-feng E-mail:lujf@sibs.ac.cn

摘要:

以斑点叉尾鮰内脏为原料,血管紧张素转化酶抑制率为指标,筛选出木瓜蛋白酶为酶解的最适蛋白酶,并研究该酶的酶解时间、酶添加量、初始pH 值、酶解温度、液料比(mL/g)对酶解产物抑制活性的影响,通过正交试验优化得到了具有ACE 抑制活性的酶解产物的最佳工艺条件。结果表明:最优酶解条件为初始pH7.5、酶解温度55℃、液料比2:1(mL/g),酶解产物ACE 抑制率为72.34%。

关键词: 斑点叉尾鮰, 鱼内脏, 抑制率, 血管紧张素转化酶, 木瓜蛋白酶

Abstract:

In this study, viscera of channel catfish, Ictalurus punctatus, were used as the raw materials to prepare angiotensin Ⅰ-converting enzyme (ACE) inhibitory products on the basis of its inhibitory rate of ACE. Papain was identified as the optimal enzyme. The effects of enzymatic hydrolysis time, enzyme addition amount, initial pH, hydrolysis temperature, and liquidmaterial ratio on the inhibitory rate of prepared ACE inhibitory products were investigated by orthogonal experiments. Results indicated that the optimal enzymatic hydrolysis conditions were initial pH of 7.5, hydrolysis temperature of 60 ℃, and liquidmaterial ratio of 2:1. Under the optimal conditions, the ACE inhibitory rate of enzymatic hydrolysate reached up to 72.34%.

Key words: Ictalurus punctatus, fish viscera, inhibitory rate, angiotensin Ⅰ -converting enzyme, papain

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