食品科学 ›› 2010, Vol. 31 ›› Issue (17): 14-17.doi: 10.7506/spkx1002-6630-201017003

• 基础研究 • 上一篇    下一篇

超临界CO2杀菌过程中萃取机制研究

周先汉1 , 2,程丽梅2,曾庆梅1,张 安2,宋俊骅2,周典飞2   

  1. 1.合肥工业大学 农产品生物化工教育部工程研究中心 2.合肥工业大学生物与食品工程学院
  • 收稿日期:2009-11-09 修回日期:2010-07-10 出版日期:2010-09-15 发布日期:2010-12-29
  • 通讯作者: 程丽梅 E-mail:chenglimeicom@163.com
  • 基金资助:

    国家自然科学基金项目(30871739;30571304);安微省教育厅重点科研项目(KJ2007A099)

Extraction Mechanism during the Sterilization of Supercritical Carbon Dioxide

ZHOU Xian-han1,2,CHENG Li-mei2,ZENG Qing-mei1,ZHANG An2,SONG Jun-hua2,ZHOU Dian-fei2   

  1. (1.Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;
    2.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
  • Received:2009-11-09 Revised:2010-07-10 Online:2010-09-15 Published:2010-12-29
  • Contact: CHENG Li-mei2 E-mail:chenglimeicom@163.com

摘要:

在相同工艺条件下对大肠杆菌菌悬液作超临界CO2 处理,对不同时间段萃取液的成分进行分析。结果表明,萃取物主要有两大类:非极性脂肪酸类及极性较弱的小分子氨基酸类物质,均为细胞的功能性物质。证实在超临界CO2 杀菌过程中确实发生了萃取作用。

关键词: 超临界CO2, 萃取, 杀菌机理, 大肠杆菌

Abstract:

Functional components in cytoplasm, cell membrane and cell wall can be dissolved by supercritical carbon dioxide. As a result, the cells will be damaged and die due to inactivation. The extraction mechanism during supercritical carbon dioxide sterilization was explored in this study. An Escherichia coli suspension at a concentration of 108 CFU/mL was prepared and treated with supercritical carbon dioxide and composition analysis was performed on the extracts obtained at different time points. The results indicated that both non-polar fatty acids and polar weak small amino acids were the functional components in cells. This investigation demonstrates that extraction indeed occurs during supercritical carbon dioxide sterilization.

Key words: supercritical carbon dioxide, extraction, sterilization mechanism, Escherichia coli

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