关键词: 猪牙花, 抑菌, 抗氧化, 微量元素

Abstract:

Objective: To evaluate whether the ethanol extract from Erythronium japonicum Decne has antibacterial activity and the ability to scavenge hydroxyl radicals and determine its trace elements. Methods: The antibacterial activity of the ethanol extract against four common bacteria was assessed by measuring its minimal inhibitory concentrations (MIC) by filter paper method and the effects of temperature, pH and UV irradiation on its antibacterial activity were dealt with. Trace elements such as iron, magnesium, zinc and copper were determined using atomic absorption spectrophotometry. Experimental results were subjected to statistical analysis using SPSS16.0. Results: The MICs of the ethanol extract were 12.5, 100, 100 mg/mL and 200mg/mL for Staphylococcus aureus, Salmonella, Bacillus subtilis and Escherichia coli, respectively. Thermal treatment, acidic environment and UV irradiation had no obvious effect on the antibacterial activity of the ethanol extract. The contents of iron, magnesium, zinc and copper of the ethanol extract were 633.25, 30.45, 39.95μg/g and 12μg/g, respectively. Conclusion: Erythronium japonicum Decne contains high levels of trace elements and exhibits excellent antibacterial and hydroxyl radical scavenging activities. Thus, this herb has a high exploitation value.

Key words: Erythronium japonicum Decne, antibacterial activity, hydroxyl radical scavenging activity, trace elements