食品科学 ›› 2010, Vol. 31 ›› Issue (10): 326-329.doi: 10.7506/spkx1002-6630-201010072

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1-甲基环丙烯处理对采后甜瓜活性氧代谢的影响

孙爱萍1,郑永华2,杨海燕1,李学文1 , 2 ,* ,韩 伟1,张喜军1   

  1. 1.新疆农业大学食品科学学院 2.南京农业大学食品科技学院
  • 收稿日期:2008-11-28 修回日期:2010-02-25 出版日期:2010-05-15 发布日期:2010-12-29
  • 通讯作者: 李学文 E-mail:xjndsp@sina.com

Effect of 1-MCP Treatment on Reactive Oxygen Metabolism and Related Enzymes in Melon during Postharvest Storage

SUN Ai-ping1,ZHENG Yong-hua2,YANG Hai-yan1,LI Xue-wen1,2,*,HAN Wei1,ZHANG Xi-jun1   

  1. 1. College of Food Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2008-11-28 Revised:2010-02-25 Online:2010-05-15 Published:2010-12-29
  • Contact: LI Xue-wen E-mail:xjndsp@sina.com

摘要:

研究1- 甲基环丙烯(1-MCP)处理对甜瓜(Cucuis melo L.ssp.melo pang)果实在贮藏过程中对其活性氧(reactive oxygen species,ROS)相关代谢的影响,在室温 (22~25℃)条件下,用1μL/L 1-MCP 处理早熟甜瓜“早黄蜜” 24h,贮藏于22℃,对果实中活性氧代谢的相关酶:超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性及超氧阴离子(O2 - ·)和丙二醛(MDA)含量进行测定。结果表明:1-MCP 处理抑制了甜瓜果实CAT 活性的下降,贮藏前期提高了SOD 活性,促进POD 活性迅速升高,并使其在第4 天达到峰值,贮藏过程高于对照果实。1-MCP 处理可显著降低果实中O2 - ·的产生速率,抑制MDA 的产生,延缓甜瓜果实采后衰老。

关键词: 1 - 甲基环丙烯, 甜瓜, 活性氧代谢

Abstract:

The fruits of Zaohuangmi melon, an early ripening melon cultivar, were treated with 1 μL/L 1-methylcyclopropene (1-MCP) for 24 h at ambient temperature (22-25 ℃) and then stored at 22 ℃. The activities of reactive oxygen species (ROS) metabolism related enzymes: SOD, POD, CAT and POD, superoxide anion radical production rate and the content of MDA in the treated melon fruits were measured periodically during 22 days of storage. The results showed that exposure of melon fruits to 1-CMP prevented the decline of CAT activity, caused the increase of SOD activity at the beginning of storage and significantly increased POD activity, reaching a peak value after 3 days. The POD activity of 1-CMP-treated melon fruits was higher than that of control fruits over the entire storage period. 1-CMP-treated melon fruits exhibited significantly lower superoxide anion radical production rate and lower MDA content and thus, the postharvest senescence of melon fruits was postponed.

Key words: 1-methylcyclopropene (1-MCP), melon fruit, reactive oxygen species (ROS) metabolism

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