食品科学 ›› 2010, Vol. 31 ›› Issue (8): 70-73.doi: 10.7506/spkx1002-6300-201008016

• 工艺技术 • 上一篇    下一篇

柚皮苷酶对琯溪蜜柚果汁脱苦效果工艺优化

黄高凌1,2,倪 辉1,2,胡 阳1,2,郭飞飞1,蔡慧农1,2,*   

  1. 1.集美大学生物工程学院 2.厦门市食品生物工程技术研究中心
  • 收稿日期:2009-05-25 出版日期:2010-04-15 发布日期:2010-12-29
  • 通讯作者: 蔡慧农 E-mail:nihui@jmu.edu.cn
  • 基金资助:

    福建省自然科学基金项目(B0610030);厦门市科技计划项目(3502Z20063016)

Optimization of Naringinase Treatment for Debittering of Citrus grandis (L.) Osbeck cv. Guanximiyou Juice

HUANG Gao-ling1,2,NI Hui1,2,HU Yang1,2,GUO Fei-fei1,CAI Hui-nong1,2   

  1. 1. College of Bioengineering, Jimei University, Xiamen 361021, China;
    2. Food Biotechnology Engineering Research Center of Xiamen, Xiamen 361021, China
  • Received:2009-05-25 Online:2010-04-15 Published:2010-12-29
  • Contact: CAI Hui-nong E-mail:nihui@jmu.edu.cn

摘要:

以琯溪蜜柚果汁为对象,研究在不同条件下柚皮苷酶对蜜柚果汁的脱苦效果。采用HPLC 测定柚皮苷含量,通过单因素试验分别考察加酶量、温度、酶解时间及pH 值对柚皮苷酶酶解反应的影响,在单因素试验基础上以柚皮苷脱除率为指标进行正交试验,确定最佳工艺条件:酶解温度60℃,酶活7.4U/mL 柚汁,酶解时间100min,pH3.6。在此条件下琯溪蜜柚果汁的柚皮苷脱除率达97% 以上。

关键词: 琯溪蜜柚, 果汁, 柚皮苷酶, 柚皮苷, 脱苦

Abstract:

In the present study, naringinase, an enzyme that hydrolyzes naringin responsible for bitter taste, was used to debitter Citrus grandis (L.) Osbeck cv. Guanximiyou (Guanxi pomelo) juice. To maximize the removal rate of naringin from Guanxi pomelo juice, major hydrolysis parameters including enzyme dosage, reaction temperature and time and pH were investigated by single factor and orthogonal array design methods. Naringin in Guanxi pomelo juice was analyzed by reversed phase high performance liquid chromatographic method. Results indicated that a maximum removal rate of naringin of more than 97% was achieved by using 7.4 U/mL of naringinase to treat Guanxi pomelo juice for 100 min at 60 ℃ and pH 3.6.

Key words: Guanxi pomelo, juice, naringinase, naringenin, debittering

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