关键词: 橙皮苷, 酸水解, 橙皮素-7-葡萄糖苷, 分离

Abstract:

To provide a method for preparing two hesperidin hydrolysates, namely hesperetin-7-glucoside and hesperetin, acidcatalyzed hydrolysis of hesperidin in an alkaline organic solvent system was explored and the resulting hesperidin hydrolysates were preliminarily separated. Crucial factors affecting acid-catalyzed hydrolysis, such as acid kind, hydrolysis mode, acid concentration, composition of alkaline organic solvent system and hydrolysis time were investigated. Results showed that full dissolution of hesperidin in a mixed system of sodium hydroxide solution and isopropyl alcohol under preheating condition followed by hydrolysis for 15－45 min catalyzed with 1.5 mol/L hydrochloric acid resulted in an optimal hesperidin hydrolysis. Moreover, a satisfying separation of 3 hydrolysis products obtained was achieved using their difference of dissolubility in acetone and water. Thus, some concentration of hydrochloric acid in an appropriate alkaline organic solvent medium can give a good catalysis for hesperidin hydrolysis.

Key words: hesperidin, acid-catalyzed hydrolysis, hesperetin-7-glucoside, separation