球等鞭金藻胞内和胞外多糖的提取工艺

doi:10.7506/spkx1002-6630-201006013

食品科学 ›› 2010, Vol. 31 ›› Issue (6): 55-59.doi: 10.7506/spkx1002-6630-201006013

球等鞭金藻胞内和胞外多糖的提取工艺

孙颖颖¹，雷琪瑶¹，刘筱潇¹，朱明¹，王长海^{2 , 3}

1.淮海工学院江苏省海洋生物技术重点实验室 2.烟台大学海洋学院 3.大连理工大学环境与生命学院

收稿日期:2009-03-31 修回日期:2009-10-26 出版日期:2010-03-15 发布日期:2010-12-29
通讯作者: 孙颖颖 E-mail:syy-999@163.com
基金资助:
淮海工学院人才引进科研启动项目(KQ08001)；连云港市科技发展计划(工业攻关)项目(CG0803-1)；
连云港市科技发展计划(社会发展)项目(SH0702)；“十一五”国家科技支撑计划重大项目(2006BAD09A01)

Extraction of Isochrysis galbana Extracellular and Intercellular Polysaccharides

SUN Ying-ying1，LEI Qi-yao1，LIU Xiao-xiao1，ZHU Ming1，WANG Chang-hai2,3

1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China；
2. School of Ocean, Yantai University, Yantai 264005, China ；
3. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China

Received:2009-03-31 Revised:2009-10-26 Online:2010-03-15 Published:2010-12-29
Contact: SUN Ying-ying1 E-mail:syy-999@163.com

摘要/Abstract

摘要：

采用热水浸提法，通过一系列单因素试验，研究提取时间、提取pH 值、提取温度和液料比对球等鞭金藻胞内和胞外多糖提取的影响。在此基础上，通过正交试验进一步优化胞内和胞外多糖的提取工艺。最后，采用适宜提取工艺制备胞内和胞外粗多糖样品，并测定该样品中蛋白质和多糖含量。单因素试验结果表明，提取时间、提取pH 值和提取温度均能显著影响胞内和胞外多糖的提取。对胞内粗多糖提取率而言，提取时间180min、提取pH9 和提取温度70℃为最适宜的提取条件；当提取时间300min、提取pH10 和提取温度80℃时，更利于胞外粗多糖的提取。液料比对胞内和胞外粗多糖提取的影响较小，20:1(mL/g)为多糖提取合适的液料比；正交试验结果表明，胞内(胞外)多糖的适宜提取工艺为提取时间、提取pH 值、提取温度和液料比分别为180min(240min)、pH8(9)、70℃和15:1。粗多糖样品的蛋白质和多糖含量分别为1.18%(0.82%)和 22.1%(18.6%)，很低的蛋白质含量和较高的多糖含量表明采用该提取工艺获得的粗多糖样品纯度较高，利于样品的后续分离纯化。

关键词: 球等鞭金藻, 胞内多糖, 胞外多糖, 热水浸提, 提取工艺

Abstract:

After 10 days of cultivation in f/2 medium under appropriate conditions, Isochrysis galbana cells and cultivation supernatant were separated by centrifugation and prepared into powders by freeze drying method for extracting intercellular and extracellular polysaccharides by hot water extraction, respectively. A series of single factor experiments was conducted to examine the effects of technological parameters such as extraction time, pH, extraction temperature and material/liquid on extraction of intercellular and extracellular polysaccharides. Subsequently, the optimal values of the parameters were determined using orthogonal array design. Finally, extracted intercellular and extracellular polysaccharides were analyzed for protein and polysaccharide contents. Single factor analysis showed that extraction time, pH and extraction temperature all had an obvious effect on extraction of intercellular and extracellular polysaccharides. For intercellular polysaccharide extraction, the optimal values of the three parameters were 180 min, 9 and 70 ℃, respectively; in contrast, an optimal extracellular polysaccharide extraction was obtained under the following parameters: pH 10 and 80 ℃ for 300 min extraction. Material/liquid ratio had little effect on extraction of intercellular and extracellular polysaccharides, and an appropriate value of 20:1 (mL/g) was found. Orthogonal array design analysis demonstrated that the optimal values of extraction time, pH and extraction temperature were 180 min, pH 8, 70 ℃ and 15:1 for intercellular polysaccharide extraction and 240 min, pH 9, 70 ℃ and 15:1 for extracellular polysaccharide extraction. The protein and polysaccharide contents in target products obtained under optimized technological parameters were 1.18% and 22.1% for crude intercellular polysaccharide and 0.82% and 18.6% for crude extracellular polysaccharide, respectively. So low protein content and so high polysaccharide content indicate high purity of target polysaccharides resulting from the orthogonal array optimization, which is favorable to further purification.

Key words: Isochrysis galbana, intracellular polysaccharides, extracellular polysaccharides, hot water extraction

中图分类号:

Q946.3

孙颖颖1，雷琪瑶1，刘筱潇1，朱明1，王长海2,3. 球等鞭金藻胞内和胞外多糖的提取工艺[J]. 食品科学, 2010, 31(6): 55-59.

SUN Ying-ying1，LEI Qi-yao1，LIU Xiao-xiao1，ZHU Ming1，WANG Chang-hai2,3. Extraction of Isochrysis galbana Extracellular and Intercellular Polysaccharides[J]. FOOD SCIENCE, 2010, 31(6): 55-59.

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球等鞭金藻胞内和胞外多糖的提取工艺

Extraction of Isochrysis galbana Extracellular and Intercellular Polysaccharides

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